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Temporal and spatial distribution of paramoebae in the water column - a pilot study


Douglas-Helders, GM and O'Brien, DP and McCorkell, BE and Zilberg, D and Gross, A and Carson, J and Nowak, BF, Temporal and spatial distribution of paramoebae in the water column - a pilot study, Journal of Fish Diseases, 26, (4) pp. 231-240. ISSN 0140-7775 (2003) [Refereed Article]

DOI: doi:10.1046/j.1365-2761.2003.00452.x


Amoebic gill disease is the main disease affecting the salmonid industry in Tasmania, but no information on the distribution of the causative pathogen, Neoparamoeba pemaquidensis, in the aquatic environment is available. This pilot study aimed to determine temporal and spatial distributions of paramoebae species in the water column, using an immuno-dot blot technique. Water samples were collected from inside fish cages at various depths (0.5, 5.5 and 11.0 m) in both summer and winter, as well as various distances (0, 0.5, 240, 280, 750 and 1100 m) away from the sea cage and farming site. Paramoebae densities were estimated using the most probable number technique (MPN). Temperature, salinity, dissolved oxygen, turbidity, nitrite and nitrates, and bacterial counts were measured for each water sample. Data were analysed using a residual maximum likelihood test and significant associations between paramoebae densities and environmental factors were analysed. Results showed that densities were significantly higher in summer (P = 0.017), at 5.5 m depth (P = 0.029), and reduced to the lowest density at 1100 m away from the cage sites (P = 0.008). Bacterial counts, turbidity and temperature were found to be significantly associated with paramoebae densities.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Douglas-Helders, GM (Dr Greetje Douglas-Helders)
UTAS Author:Carson, J (Dr Jeremy Carson)
UTAS Author:Nowak, BF (Professor Barbara Nowak)
ID Code:26805
Year Published:2003
Web of Science® Times Cited:27
Deposited By:TAFI - Aquaculture
Deposited On:2003-08-01
Last Modified:2004-03-29

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