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Role of Eucalyptus globulus wound wood extractives: evidence of superoxide dismutase-like activity

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posted on 2023-05-16, 14:08 authored by Alieta EylesAlieta Eyles, Noel DaviesNoel Davies, Mitsunaga, T, Mihara, R, Caroline MohammedCaroline Mohammed
Superoxide dismutase-like activity of crude Eucalyptus globulus extracts obtained from new wound wood was measured using the water-soluble tetrazolium salt (WST)-1 assay. This in vitro assay determined the activity of superoxide radicals scavenging in a xanthine oxidase system. The 50% inhibition (IC 50) of formazan formation was estimated for fractionated samples and purified compounds isolated from the crude wound wood extract, i.e. engelitin and pedunculagin. Standards of gallic acid and pentagalloyl glucose were also measured. Fraction II as well as the compounds pentagalloyl glucose and pedunculagin showed the highest levels of antioxidant activity. Subsequent analysis of fraction II by high-performance liquid chromatography (HPLC) coupled with negative ion electrospray mass spectrometry revealed the fraction to be dominated by hydrolysable tannins including pedunculagin, di-, tri- and tetragalloylglucoses. These preliminary results provide some evidence for an antioxidant role of secondary metabolites in tree wound repair and defence.

History

Publication title

Forest Pathology

Volume

34

Issue

4

Pagination

225-232

ISSN

1437-4781

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Blackwell Verlag GMBH

Place of publication

Berlin, Germany

Repository Status

  • Restricted

Socio-economic Objectives

Hardwood plantations

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