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Role of Eucalyptus globulus wound wood extractives: evidence of superoxide dismutase-like activity


Eyles, A and Davies, NW and Mitsunaga, T and Mihara, R and Mohammed, CL, Role of Eucalyptus globulus wound wood extractives: evidence of superoxide dismutase-like activity, Forest Pathology, 34, (4) pp. 225-232. ISSN 1437-4781 (2004) [Refereed Article]

DOI: doi:10.1111/j.1439-0329.2004.00361.x


Superoxide dismutase-like activity of crude Eucalyptus globulus extracts obtained from new wound wood was measured using the water-soluble tetrazolium salt (WST)-1 assay. This in vitro assay determined the activity of superoxide radicals scavenging in a xanthine oxidase system. The 50% inhibition (IC 50) of formazan formation was estimated for fractionated samples and purified compounds isolated from the crude wound wood extract, i.e. engelitin and pedunculagin. Standards of gallic acid and pentagalloyl glucose were also measured. Fraction II as well as the compounds pentagalloyl glucose and pedunculagin showed the highest levels of antioxidant activity. Subsequent analysis of fraction II by high-performance liquid chromatography (HPLC) coupled with negative ion electrospray mass spectrometry revealed the fraction to be dominated by hydrolysable tannins including pedunculagin, di-, tri- and tetragalloylglucoses. These preliminary results provide some evidence for an antioxidant role of secondary metabolites in tree wound repair and defence.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Forestry sciences
Research Field:Agroforestry
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood plantations
UTAS Author:Eyles, A (Dr Alieta Eyles)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
UTAS Author:Mohammed, CL (Professor Caroline Mohammed)
ID Code:26652
Year Published:2004
Web of Science® Times Cited:18
Deposited By:Agricultural Science
Deposited On:2004-08-01
Last Modified:2005-06-04

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