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Effect of extended-spectrum -lactamases on the susceptibility of Haemophilus influenzae to cephalosporins


Tristram, SG, Effect of extended-spectrum a-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins, Journal of Antimicrobial Chemotherapy, 51, (1) pp. 39-43. ISSN 0305-7453 (2003) [Refereed Article]

DOI: doi:10.1093/jac/dkg015


The extended-spectrum β-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and 0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are higher than those of β-lactamase-negative strains, or those producing simple wild-type TEM-1 β-lactamase, but not high enough to be categorized as resistant according to the breakpoints of the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion methodology and interpretive criteria. This study shows that current NCCLS susceptibility testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Tristram, SG (Dr Stephen Tristram)
ID Code:26362
Year Published:2003
Web of Science® Times Cited:11
Deposited By:Health Sciences A
Deposited On:2003-08-01
Last Modified:2010-06-04

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