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Production of exopolysaccharides by Antarctic marine bacterial isolates


Mancuso Nichols, CA and Garon, S and Bowman, JP and Raguenes, G and Guezennec, J, Production of exopolysaccharides by Antarctic marine bacterial isolates, Journal of Applied Microbiology, 96, (5) pp. 1057-1066. ISSN 1364-5072 (2004) [Refereed Article]

DOI: doi:10.1111/j.1365-2672.2004.02216.x


Aims: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. Methods and Results: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10°C than at 20°C. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. Conclusions: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. Significance and Impact of the Study: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Environmental Management
Objective Group:Marine systems and management
Objective Field:Oceanic processes (excl. in the Antarctic and Southern Ocean)
UTAS Author:Mancuso Nichols, CA (Dr Carol Mancuso Nichols)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
ID Code:26268
Year Published:2004
Web of Science® Times Cited:147
Deposited By:Agricultural Science
Deposited On:2004-08-01
Last Modified:2011-10-12

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