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Molecular identification of uncoupling proteins (UCP2 and UCP3) and absence of UCP1 in the marsupial Tasmanian bettong, Bettongia gaimardi

Citation

Kabat, AP and Rose, RW and Harris, JA and West, AK, Molecular identification of uncoupling proteins (UCP2 and UCP3) and absence of UCP1 in the marsupial Tasmanian bettong, Bettongia gaimardi, Comparative Biochemistry and Physiology Part B - Biochemistry & Molecular Biology, 134, (1) pp. 71-77. ISSN 1096-4959 (2003) [Refereed Article]

DOI: doi:10.1016/S1096-4959(02)00189-6

Abstract

This study has identified the expression of uncoupling proteins in a marsupial using molecular techniques. The Tasmanian bettong, Bettongia gaimardi, increases non-shivering thermogenesis (NST) in response to cold exposure and norepinephrine, although previous studies have been unable to demonstrate the presence of brown adipose tissue or uncoupling protein 1 (UCP1). This study used molecular techniques to confirm the absence of UCP1 as well as ascertain if this species expresses UCP2 and/or UCP3. Tissue samples from four B. gaimardi were taken prior to and post-cold exposure at 4-5°C for 2 weeks. The tissues were then examined for UCP1, UCP2 and UCP3 expression using Western blotting. UCP2 and UCP3 were amplified through RT-PCR and subsequently sequenced to confirm molecular identity. Our work confirms that B. gaimardi does not express UCP1 and that this species expresses both uncoupling proteins 2 and 3. The sequencing of the amplified B. gaimardi UCP2 and UCP3 cDNAs have revealed a 74% homology with rat UCP2 cDNA, and 65% homology with rat UCP3 cDNA. Although this work has not yet characterised the functional properties of these proteins in the marsupial, it does suggest a possible mechanism to explain the existence of NST in B. gaimardi. © 2002 Elsevier Science Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Physiology
Research Field:Comparative Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Kabat, AP (Mr Alexander Kabat)
Author:Rose, RW (Associate Professor Randy Rose)
Author:Harris, JA (Dr Julie Harris)
Author:West, AK (Professor Adrian West)
ID Code:26260
Year Published:2003
Web of Science® Times Cited:16
Deposited By:Zoology
Deposited On:2003-08-01
Last Modified:2004-04-07
Downloads:0

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