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Neurofilament triplet proteins are restricted to a subset of neurons in the rat neocortex

journal contribution
posted on 2023-05-16, 13:58 authored by Matthew KirkcaldieMatthew Kirkcaldie, Tracey DicksonTracey Dickson, Carolyn KingCarolyn King, Grasby, DJ, Riederer, BM, James VickersJames Vickers
The cellular localisation of neurofilament triplet subunits was investigated in the rat neocortex. A subset of mainly pyramidal neurons showed colocalisation of subunit immunolabelling throughout the neocortex, including labelling with the antibody SMI32, which has been used extensively in other studies of the primate cortex as a selective cellular marker. Neurofilament-labelled neurons were principally localised to two or three cell layers in most cortical regions, but dramatically reduced labelling was present in areas such as the perirhinal cortex, anterior cingulate and a strip of cortex extending from caudal motor regions through the medial parietal region to secondary visual areas. However, quantitative analysis demonstrated a similar proportion (10-20%) of cells with neurofilament triplet labelling in regions of high or low labelling. Combining retrograde tracing with immunolabelling showed that cellular content of the neurofilament proteins was not correlated with the length of projection. Double labelling immunohistochemistry demonstrated that neurofilament content in axons was closely associated with myelination. Analysis of SMI32 labelling in development indicated that content of this epitope within cell bodies was associated with relatively late maturation, between postnatal days 14 and 21. This study is further evidence of a cell type-specific regulation of neurofilament proteins within neocortical neurons. Neurofilament triplet content may be more closely related to the degree of myelination, rather than the absolute length, of the projecting axon. Crown Copyright © 2002 Published by Elsevier Science B.V. All rights reserved.

History

Publication title

Journal of Chemical Neuroanatomy

Volume

24

Pagination

163-171

ISSN

0891-0618

Department/School

Tasmanian School of Medicine

Publisher

Elsevier

Place of publication

Amsterdam

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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