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Neurofilament triplet proteins are restricted to a subset of neurons in the rat neocortex

Citation

Kirkcaldie, MTK and Dickson, TC and King, CE and Grasby, DJ and Riederer, BM and Vickers, JC, Neurofilament triplet proteins are restricted to a subset of neurons in the rat neocortex, Journal of Chemical Neuroanatomy, 24, (3) pp. 163-171. ISSN 0891-0618 (2002) [Refereed Article]

DOI: doi:10.1016/S0891-0618(02)00043-1

Abstract

The cellular localisation of neurofilament triplet subunits was investigated in the rat neocortex. A subset of mainly pyramidal neurons showed colocalisation of subunit immunolabelling throughout the neocortex, including labelling with the antibody SMI32, which has been used extensively in other studies of the primate cortex as a selective cellular marker. Neurofilament-labelled neurons were principally localised to two or three cell layers in most cortical regions, but dramatically reduced labelling was present in areas such as the perirhinal cortex, anterior cingulate and a strip of cortex extending from caudal motor regions through the medial parietal region to secondary visual areas. However, quantitative analysis demonstrated a similar proportion (10-20%) of cells with neurofilament triplet labelling in regions of high or low labelling. Combining retrograde tracing with immunolabelling showed that cellular content of the neurofilament proteins was not correlated with the length of projection. Double labelling immunohistochemistry demonstrated that neurofilament content in axons was closely associated with myelination. Analysis of SMI32 labelling in development indicated that content of this epitope within cell bodies was associated with relatively late maturation, between postnatal days 14 and 21. This study is further evidence of a cell type-specific regulation of neurofilament proteins within neocortical neurons. Neurofilament triplet content may be more closely related to the degree of myelination, rather than the absolute length, of the projecting axon. Crown Copyright © 2002 Published by Elsevier Science B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Central Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Kirkcaldie, MTK (Dr Matthew Kirkcaldie)
Author:Dickson, TC (Professor Tracey Dickson)
Author:King, CE (Dr Carolyn King)
Author:Grasby, DJ (Mr Dallas Grasby)
Author:Vickers, JC (Professor James Vickers)
ID Code:26048
Year Published:2002
Web of Science® Times Cited:51
Deposited By:Pathology
Deposited On:2002-08-01
Last Modified:2011-10-24
Downloads:0

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