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Comparison of mitochondrial and nuclear DNA analyses of population structure in blacklip abalone Haliotis rubra Leach

Citation

Holman, N and Bartlett, JP and Evans, BS and Elliott, NG, Comparison of mitochondrial and nuclear DNA analyses of population structure in blacklip abalone Haliotis rubra Leach, Marine and Freshwater Research, 53, (3) pp. 711-718. ISSN 1323-1650 (2002) [Refereed Article]

DOI: doi:10.1071/MF01197

Abstract

Genetic variation in five geographically isolated samples of the blacklip abalone, Haliotis rubra, from south-eastern Australia was examined using two molecular techniques. A restriction fragment length polymorphism (RFLP) analysis using six restriction enzymes on the ND3/COIII region of mitochondrial DNA was compared with five independent nuclear DNA microsatellite loci. The results from both techniques suggest restricted gene flow between blacklip abalone separated by Bass Strait, and homogeneity among geographically isolated samples from around the island of Tasmania. Although both techniques showed similar resolving power, microsatellite DNA analysis is the preferred molecular technique for the fine scale investigation of blacklip abalone population structure because it makes possible the examination of numerous independent loci with potentially high levels of polymorphism. Both sample and locus specific homozygote excesses were recorded for the microsatellite loci. The most likely explanation for the locus specific deviations from Hardy-Weinberg expectations is the presence of null alleles.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Marine Flora, Fauna and Biodiversity
Author:Holman, N (Mrs Natalie Holman)
Author:Evans, BS (Mr Bradley Scott Evans)
ID Code:25632
Year Published:2002
Web of Science® Times Cited:23
Deposited By:Zoology
Deposited On:2002-08-01
Last Modified:2011-11-18
Downloads:0

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