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Microsomal metabolism and enzyme kinetics of the terpene p-cymene in the common brushtail possum (Trichosurus vulpecula), koala (Phascolarctos cinereus) and rat

Citation

Pass, GJ and McLean, SR and Stupans, I and Davies, NW, Microsomal metabolism and enzyme kinetics of the terpene p-cymene in the common brushtail possum (Trichosurus vulpecula), koala (Phascolarctos cinereus) and rat, Xenobiotica, 32, (5) pp. 383-397. ISSN 0049-8254 (2002) [Refereed Article]


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The definitive published version is available online at: http://www.tandf.co.uk/journals

Official URL: http://www.tandf.co.uk/journals

DOI: doi:10.1080/00498250210124138

Abstract

1. p-Cymene is an aromatic monoterpene found in the leaves of Eucalyptus spp. and is ingested in the diet of two marsupial folivores, the brushtail possum (Trichosurus vulpecula) and koala (Phascolarctos cinereus). The metabolism of p-cymene by liver microsomes from the possum, koala and rat (an animal whose diet is not terpene-rich) was examined.

2. The major p-cymene metabolite in each species was cuminyl alcohol, with smaller amounts of other side-chain alcohols being formed. No phenolic metabolites were detected. Possum and koala microsomes further oxidized cuminyl alcohol to cumic acid.

3. Pretreatment with a terpene diet more than doubled the Vmax for cuminyl alcohol formation by possum microsomes. This is similar to a previous finding by our group with 1,8-cineole and indicates that a terpene-containing diet results in induction of the enzymes responsible for terpene metabolism.

4. The rank order of the ability to metabolize p-cymene, measured by intrinsic clearance (Clint = Vmax/Km [µl mg protein−1 min−1]) was: terpene-treated possum (128)>control possum (107)>koala (69)>rat (38).

5. The findings support the hypothesis that animals that have adapted to a diet of Eucalypus leaf have a greater capacity than generalist feeders to metabolize dietary terpenes.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Terrestrial Ecology
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Forest and Woodlands Flora, Fauna and Biodiversity
Author:Pass, GJ (Ms Georgia Jane Pass)
Author:McLean, SR (Professor Stuart McLean)
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:25554
Year Published:2002
Web of Science® Times Cited:19
Deposited By:Pharmacy
Deposited On:2002-08-01
Last Modified:2015-07-31
Downloads:0

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