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Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula)
Citation
Boyle, RR and McLean, SR and Brandon, S and Pass, GJ and Davies, NW, Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula), Journal of Chromatography B, 780, (2) pp. 397-406. ISSN 1570-0232 (2002) [Refereed Article]
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DOI: doi:10.1016/S1570-0232(02)00625-6
Abstract
We have developed two solid-phase microextraction (SPME) methods, coupled with gas chromatography, for quantitatively analysing the major Eucalyptus leaf terpene, 1,8-cineole, in both expired air and blood from the common brushtail possum (Trichosurus vulpecula). In-line SPME sampling (5 min at 20 °C room temperature) of excurrent air from an expiratory chamber containing a possum dosed orally with 1,8-cineole (50 mg/kg) allowed real-time semi-quantitative measurements reflecting 1,8-cineole blood concentrations. Headspace SPME using 50 ìl whole blood collected from possums dosed orally with 1,8-cineole (30 mg/kg) resulted in excellent sensitivity (quantitation limit 1 ng/ml) and reproducibility. Blood concentrations ranged between 1 and 1380 ng/ml. Calibration curves were prepared for two concentration ranges (0.05–10 and 10–400 ng/50 ìl) for the analysis of blood concentrations. Both calibration curves were linear (r2=0.999 and 0.994, respectively) and the equations for the two concentration ranges were consistent.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Trichosurus vulpecula; Breath analysis |
| Research Division: | Biological Sciences |
| Research Group: | Ecology |
| Research Field: | Terrestrial Ecology |
| Objective Division: | Environment |
| Objective Group: | Flora, Fauna and Biodiversity |
| Objective Field: | Forest and Woodlands Flora, Fauna and Biodiversity |
| Author: | Boyle, RR (Dr Rebecca Boyle) |
| Author: | McLean, SR (Professor Stuart McLean) |
| Author: | Brandon, S (Mrs Susan Brandon) |
| Author: | Pass, GJ (Ms Georgia Jane Pass) |
| Author: | Davies, NW (Associate Professor Noel Davies) |
| ID Code: | 25552 |
| Year Published: | 2002 |
| Web of Science® Times Cited: | 20 |
| Deposited By: | Pharmacy |
| Deposited On: | 2002-08-01 |
| Last Modified: | 2011-05-30 |
| Downloads: | 0 |
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