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Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula)

Citation

Boyle, RR and McLean, SR and Brandon, S and Pass, GJ and Davies, NW, Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula), Journal of Chromatography B, 780, (2) pp. 397-406. ISSN 1570-0232 (2002) [Refereed Article]


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DOI: doi:10.1016/S1570-0232(02)00625-6

Abstract

We have developed two solid-phase microextraction (SPME) methods, coupled with gas chromatography, for quantitatively analysing the major Eucalyptus leaf terpene, 1,8-cineole, in both expired air and blood from the common brushtail possum (Trichosurus vulpecula). In-line SPME sampling (5 min at 20 C room temperature) of excurrent air from an expiratory chamber containing a possum dosed orally with 1,8-cineole (50 mg/kg) allowed real-time semi-quantitative measurements reflecting 1,8-cineole blood concentrations. Headspace SPME using 50 l whole blood collected from possums dosed orally with 1,8-cineole (30 mg/kg) resulted in excellent sensitivity (quantitation limit 1 ng/ml) and reproducibility. Blood concentrations ranged between 1 and 1380 ng/ml. Calibration curves were prepared for two concentration ranges (0.0510 and 10400 ng/50 l) for the analysis of blood concentrations. Both calibration curves were linear (r2=0.999 and 0.994, respectively) and the equations for the two concentration ranges were consistent.

Item Details

Item Type:Refereed Article
Keywords:Trichosurus vulpecula; Breath analysis
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Terrestrial Ecology
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Forest and Woodlands Flora, Fauna and Biodiversity
Author:Boyle, RR (Dr Rebecca Boyle)
Author:McLean, SR (Professor Stuart McLean)
Author:Brandon, S (Mrs Susan Brandon)
Author:Pass, GJ (Ms Georgia Jane Pass)
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:25552
Year Published:2002
Web of Science® Times Cited:20
Deposited By:Pharmacy
Deposited On:2002-08-01
Last Modified:2011-05-30
Downloads:0

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