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Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula)

journal contribution
posted on 2023-05-16, 13:55 authored by Boyle, RR, Stuart McLeanStuart McLean, Brandon, S, Pass, GJ, Noel DaviesNoel Davies
We have developed two solid-phase microextraction (SPME) methods, coupled with gas chromatography, for quantitatively analysing the major Eucalyptus leaf terpene, 1,8-cineole, in both expired air and blood from the common brushtail possum (Trichosurus vulpecula). In-line SPME sampling (5 min at 20 °C room temperature) of excurrent air from an expiratory chamber containing a possum dosed orally with 1,8-cineole (50 mg/kg) allowed real-time semi-quantitative measurements reflecting 1,8-cineole blood concentrations. Headspace SPME using 50 ìl whole blood collected from possums dosed orally with 1,8-cineole (30 mg/kg) resulted in excellent sensitivity (quantitation limit 1 ng/ml) and reproducibility. Blood concentrations ranged between 1 and 1380 ng/ml. Calibration curves were prepared for two concentration ranges (0.05–10 and 10–400 ng/50 ìl) for the analysis of blood concentrations. Both calibration curves were linear (r2=0.999 and 0.994, respectively) and the equations for the two concentration ranges were consistent.

History

Publication title

Journal of Chromatography B

Volume

780

Pagination

397-406

ISSN

1570-0232

Department/School

School of Pharmacy and Pharmacology

Publisher

Elsevier Science BV

Place of publication

Amsterdam, Netherlands

Rights statement

The definitive version is available at http://www.sciencedirect.com

Repository Status

  • Restricted

Socio-economic Objectives

Terrestrial biodiversity

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