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We have developed two solid-phase microextraction (SPME) methods, coupled with gas chromatography, for quantitatively analysing the major Eucalyptus leaf terpene, 1,8-cineole, in both expired air and blood from the common brushtail possum (Trichosurus vulpecula). In-line SPME sampling (5 min at 20 °C room temperature) of excurrent air from an expiratory chamber containing a possum dosed orally with 1,8-cineole (50 mg/kg) allowed real-time semi-quantitative measurements reflecting 1,8-cineole blood concentrations. Headspace SPME using 50 ìl whole blood collected from possums dosed orally with 1,8-cineole (30 mg/kg) resulted in excellent sensitivity (quantitation limit 1 ng/ml) and reproducibility. Blood concentrations ranged between 1 and 1380 ng/ml. Calibration curves were prepared for two concentration ranges (0.05–10 and 10–400 ng/50 ìl) for the analysis of blood concentrations. Both calibration curves were linear (r2=0.999 and 0.994, respectively) and the equations for the two concentration ranges were consistent.

Item Type:	Refereed Article
Keywords:	Trichosurus vulpecula; Breath analysis
Research Division:	Biological Sciences
Research Group:	Ecology
Research Field:	Terrestrial ecology
Objective Division:	Environmental Management
Objective Group:	Terrestrial systems and management
Objective Field:	Terrestrial biodiversity
UTAS Author:	Boyle, RR (Dr Rebecca Boyle)
UTAS Author:	McLean, SR (Professor Stuart McLean)
UTAS Author:	Brandon, S (Mrs Susan Brandon)
UTAS Author:	Pass, GJ (Ms Georgia Jane Pass)
UTAS Author:	Davies, NW (Associate Professor Noel Davies)
ID Code:	25552
Year Published:	2002
Web of Science® Times Cited:	23
Deposited By:	Pharmacy
Deposited On:	2002-08-01
Last Modified:	2011-05-30
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