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Comparison of aqueous and nonaqueous carrier electrolytes for the separation of penicillin V and related substances by capillary electrophoresis with UV and mass spectrometric detection

Citation

Hilder, EF and Klampfl, CW and Buchberger, W and Haddad, PR, Comparison of aqueous and nonaqueous carrier electrolytes for the separation of penicillin V and related substances by capillary electrophoresis with UV and mass spectrometric detection, Electrophoresis, 23, (3) pp. 414-420. ISSN 0173-0835 (2002) [Refereed Article]


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The definitive published version is available online at: http://interscience.wiley.com

Official URL: http://interscience.wiley.com

DOI: doi:10.1002/1522-2683(200202)23:3<414::AID-ELPS414>3.0.CO;2-K

Abstract

A method for the determination of penicillin V together with its impurities and by-products formed during biosynthesis, using capillary electrophoresis (CE) with UV and electrospray-mass spectrometric (ESI-MS) detection is presented. Aqueous and nonaqueous electrolytes containing 20 mM ammonium acetate were investigated to determine their suitability for the separation of these analytes. These carrier electrolytes were optimized with respect to the pH and the solvent/s used (water, methanol, acetonitrile, ethanol and isopropanol) and it was shown that although the nonaqueous electrolytes offered unique separation selectivities, the best results in terms of selectivity and sensitivity were obtained for the aqueous system. Finally, the applicability of this method for the analysis of a mixture representative of a real fermentation broth was demonstrated using an aqueous carrier electrolyte with both UV and ESI-MS detection.

Item Details

Item Type:Refereed Article
Keywords:Capillary electrophoresis; Electrospray-mass spectrometric detection; Penicillin
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Haddad, PR (Professor Paul Haddad)
ID Code:25319
Year Published:2002
Web of Science® Times Cited:31
Deposited By:Chemistry
Deposited On:2002-08-01
Last Modified:2010-02-05
Downloads:1 View Download Statistics

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