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Rapid ion chromatography of L-ascorbic acid, nitrite, sulfite, oxalate, iodide and thiosulfate by isocratic elution utilizing a postcolumn reaction with cerium(IV) and fluorescence detection

Citation

Miura, Y and Hatakeyama, M and Hosino, T and Haddad, PR, Rapid ion chromatography of L-ascorbic acid, nitrite, sulfite, oxalate, iodide and thiosulfate by isocratic elution utilizing a postcolumn reaction with cerium(IV) and fluorescence detection, Journal of Chromatography A, 956, (1-2) pp. 77-84. ISSN 0021-9673 (2002) [Refereed Article]


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DOI: doi:10.1016/S0021-9673(01)01497-2

Abstract

Rapid separation and determination of mixtures of Image -ascorbic acid, nitrite, sulfite, oxalate, iodide and thiosulfate by conventional ion chromatography is often difficult due to incomplete separation of Image -ascorbic acid and nitrite from the water peak when using eluents giving short elution times for iodide and thiosulfate. Separation of the six species within about 15 min has been achieved by isocratic elution using a resin-based ion-exchange column with a carbonate eluent containing a trace amount of 1,3,5-benzenetricarboxylic acid (BTA) and fluorescence measurement of cerium(III) formed via postcolumn reactions of the separated sample species with cerium(IV). Calibration plots of peak height versus concentration were linear up to 10.0 μM (1.76 ppm) for Image -ascorbic acid, 8.0 μM (0.37 ppm) for nitrite, 8.0 μM (0.70 ppm) for oxalate, 80.0 μM (10.2 ppm) for iodide and 25.0 μM (2.80 ppm) for thiosulfate, whilst the sulfite calibration was linear up to 25.0 μM (2.00 ppm) when peak area was plotted against concentration. Detection limits (defined as S/N=3) were 18 ppb for Image -ascorbic acid, 4 ppb for nitrite, 16 ppb for sulfite, 7 ppb for oxalate, 72 ppb for iodide and 37 ppb for thiosulfate. The proposed method was applied successfully to the determination of Image -ascorbic acid, nitrite, sulfite, oxalate, iodide or thiosulfate in water samples.

Item Details

Item Type:Refereed Article
Keywords:Ascorbic acid; Nitrite; Sulfite; Oxalate; Iodide; Thiosulfate; Inorganic anions; Organic acids; Cerium; Benzenetricarboxylic acid
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Haddad, PR (Professor Paul Haddad)
ID Code:25312
Year Published:2002
Web of Science® Times Cited:37
Deposited By:Chemistry
Deposited On:2002-08-01
Last Modified:2010-02-08
Downloads:0

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