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Vacancy ion-exclusion chromatography of carboxylic acids on a weakly acidic cation-exchange resin


Tanaka, K and Ding, MY and Helaleh, MIH and Taoda, H and Takahashi, H and Hu, W and Hasebe, K and Haddad, PR and Fritz, J and Sarzanini, C, Vacancy ion-exclusion chromatography of carboxylic acids on a weakly acidic cation-exchange resin, Journal of Chromatography A, 956, (1-2) pp. 209-214. ISSN 0021-9673 (2002) [Refereed Article]

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DOI: doi:10.1016/S0021-9673(02)00393-X


In this preliminary study, a new approach to ion-exclusion chromatography is proposed to overcome the relatively poor conductivity detection response which occurs in ion-exclusion chromatography when acids are added to the eluent in order to improve peak shape. This approach, termed vacancy ion-exclusion chromatography, requires the sample to be used as eluent and a sample of water to be injected onto a weakly acidic cation-exchange column (TSKgel OApak-A). Vacancy peaks for each of the analytes appear at the retention times of these analytes. Highly sensitive conductivity detection is possible and sharp, well-shaped peaks are produced, leading to efficient separations. Retention times were found to be affected by the concentration of the analytes in the eluent, and also by the presence of an organic modifier such as methanol in the eluent. Detection limits for oxalic, formic, acetic, propionic, butyric and valeric acids were 0.1, 0.2, 0.3, 0.3, 0.4 and 0.5 μM, respectively, and linear ranges for some acids extended over two orders of magnitude. Precision values for retention times were 0.21% and for peak areas were <1.90%. The vacancy ion-exclusion chromatography method was found to give detection responses four to 10 times higher than conventional ion-exclusion chromatography using sulfuric acid eluent and two to five times higher than conventional ion-exclusion chromatography using benzoic acid eluent.

Item Details

Item Type:Refereed Article
Keywords:vacancy chromatography, ion-exclusion chromatography, carboxylic acids
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:25310
Year Published:2002
Web of Science® Times Cited:29
Deposited By:Chemistry
Deposited On:2002-08-01
Last Modified:2012-10-18

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