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Vacancy ion-exclusion chromatography of carboxylic acids on a strongly acidic cation-exchange resin


Tanaka, K and Ding, MY and Takahashi, H and Helaleh, MIH and Taoda, H and Hu, W and Hasebe, K and Haddad, PR and Mori, M and Fritz, JS and Sarzanini, C, Vacancy ion-exclusion chromatography of carboxylic acids on a strongly acidic cation-exchange resin, Analytica Chimica Acta, 474, (1-2) pp. 31-35. ISSN 0003-2670 (2002) [Refereed Article]

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DOI: doi:10.1016/S0003-2670(02)01012-7


A new approach to ion-exclusion chromatography (IEC) is proposed to overcome the relatively poor conductivity detection response which occurs in IEC on when acids are added to the eluent in order to improve peak shape. This approach, termed vacancy IEC, requires the sample to be used as mobile phase and a sample of water to be injected onto a polystyrene/divinylbenzene (PS/DVB)-based strongly acidic cation-exchange column (TSKgel SCX). Vacancy peaks for each of the analytes appear at the retention times of these analytes. Highly sensitive conductivity detection is possible and sharp, well-shaped peaks are produced, leading to efficient separations. Retention times were found to be affected by the concentration of the analytes in the eluent, and also by the presence of an organic modifier, such as butanol in the mobile phase. Detection limits for oxalic, formic, acetic, propionic, butyric and valeric acids at S/N=3 were 0.04, 0.1, 0.47, 0.66, 0.71 and 0.82 μM, respectively, and linear ranges for some acids extended over two-orders of magnitude. Precision values for retention times were <0.57% and for peak height were <2.06%.

Item Details

Item Type:Refereed Article
Keywords:Ion-exclusion chromatography; Strongly acidic cation-exchange resin; Vacancy chromatography; Aliphatic carboxylic acids; Conductimetric detection
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:25297
Year Published:2002
Web of Science® Times Cited:20
Deposited By:Chemistry
Deposited On:2002-08-01
Last Modified:2010-02-08

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