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The bushy pea mutant is IAA-deficient


Symons, GM and Ross, JJ and Murfet, IC, The bushy pea mutant is IAA-deficient, Physiologia Plantarum, 116, (3) pp. 389-397. ISSN 0031-9317 (2002) [Refereed Article]

DOI: doi:10.1034/j.1399-3054.2002.1160315.x


The bushy mutant of pea (Pisum sativum L.) is characterized by short, thin stems, very small leaves and profuse branching. The bushy phenotype is conferred by a dominant allele, termed bsh. Here we show that bushy plants contain lower levels of free indole-3-acetic acid (IAA) than wild-type (WT) plants. On emergence from the growth medium, bushy seedlings do not immediately display the mutant phenotype, and the effect of bsh on IAA content is small. After 10-14 days, the bushy phenotype begins to develop, and while the IAA content rises in WT plants, it falls in the mutant. The resulting difference in IAA level between WT and mutant can be up to 12-fold. Although there is a deficiency of free IAA in bushy plants, the total IAA content (including free and conjugated forms) was not reduced in comparison with the WT. Furthermore, in bushy plants, the level of the main IAA conjugate in pea, IAAsp, was not reduced to the same extent as that of IAA, and metabolism studies indicated that faster IAA deactivation might contribute to IAA deficiency in the mutant. Application of IAA to bushy plants did not result in a WT phenocopy. However, the short internodes and profuse branching of bushy plants is consistent with classical views on how IAA affects plant development.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Symons, GM (Mr Gregory Symons)
UTAS Author:Ross, JJ (Associate Professor John Ross)
UTAS Author:Murfet, IC (Professor Ian Murfet)
ID Code:25179
Year Published:2002
Web of Science® Times Cited:21
Deposited By:Plant Science
Deposited On:2002-08-01
Last Modified:2011-08-02

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