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Control of gibberellin levels and gene expression during de-etiolation in pea


Reid, JB and Botwright, NA and Smith, JJ and O'Neill, DP and Kerckhoffs, LH, Control of gibberellin levels and gene expression during de-etiolation in pea, Plant Physiology, 128, (2) pp. 734-741. ISSN 0032-0889 (2002) [Refereed Article]

DOI: doi:10.1104/pp.010607


Gibberellin A1 (GA1) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GA8 levels are increased in response to 4 h of red light, whereas the levels of GA19, GA20, and GA29 do not vary substantially. Red light appears to control GA1 levels by down-regulating the expression of Mendel's LE (PsGA3ox1) gene that controls the conversion of GA20 to GA1, and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GA1 to GA8. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20ox1, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA3ox1 shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Reid, JB (Professor Jim Reid)
UTAS Author:Botwright, NA (Miss Natasha Botwright)
UTAS Author:Smith, JJ (Ms Jenny Smith)
UTAS Author:O'Neill, DP (Mr Damian O'Neill)
UTAS Author:Kerckhoffs, LH (Dr Leonardus Kerckhoffs)
ID Code:25138
Year Published:2002
Web of Science® Times Cited:59
Deposited By:Plant Science
Deposited On:2002-08-01
Last Modified:2010-11-08

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