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Sheep have an unusual variant of the brain-specific metallothionein, metallothionein-III


Chung, RS and Holloway, AF and Eckhardt, BL and Harris, JA and Vickers, JC and Chuah, MI and West, AK, Sheep have an unusual variant of the brain-specific metallothionein, metallothionein-III, Biochemical Journal, 365 pp. 323-328. ISSN 0264-6021 (2002) [Refereed Article]

DOI: doi:10.1042/BJ20011751


Sheep metallothionein-III (MT-III) cDNA was isolated from a brain cDNA library and characterized. In contrast with MT-III from other species, sheep MT-III cDNA is predicted to encode a protein with significantly different metal-binding properties, owing to the loss of three of its cysteine residues. RT-PCR from other sheep confirmed that this aberrant structure is ubiquitous in this species. MT-III was successfully isolated from sheep brain, demonstrating that the cDNA does give rise to a protein product of the predicted structure. Sheep MT-III is similar to other mammalian MT-IIIs in that it retains the Cys-Pro-Cys-Pro motif which is thought to encode growth-inhibitory activity, and we show that it is likewise able to inhibit neuron survival in vitro. This is the first naturally occurring variant of MT-III (or any other major mammalian MT gene) which gives rise to a protein product. These findings are discussed in light of proposed roles of MT in the mammalian brain.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Biochemistry and cell biology not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Chung, RS (Associate Professor Roger Chung)
UTAS Author:Holloway, AF (Professor Adele Holloway)
UTAS Author:Eckhardt, BL (Mr Bedrich Eckhardt)
UTAS Author:Harris, JA (Dr Julie Harris)
UTAS Author:Vickers, JC (Professor James Vickers)
UTAS Author:Chuah, MI (Associate Professor Inn Chuah)
UTAS Author:West, AK (Professor Adrian West)
ID Code:24852
Year Published:2002
Web of Science® Times Cited:18
Deposited By:Biochemistry
Deposited On:2002-08-01
Last Modified:2011-09-27

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