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Nutritive blood flow improves interstitial glucose and lactate exchange in perfused rat hindlimb


Newman, JMB and Rattigan, S and Clark, MG, Nutritive blood flow improves interstitial glucose and lactate exchange in perfused rat hindlimb, American Journal of Physiology - Heart and Circulatory Physiology, 283, (1) pp. H186-H192. ISSN 0363-6135 (2002) [Refereed Article]

DOI: doi:10.1152/ajpheart.01024.2001


Microdialysis was used to assess the interstitial concentrations of glucose and lactate in the constant-flow-perfused rat hindlimb under varying levels of nutritive flow controlled by vasoconstrictors. Increased nutritive flow was achieved by norepinephrine (NE) or angiotensin II (ANG II) and decreased nutritive flow by serotonin (5-HT). NE and ANG II increased oxygen and glucose uptake as well as hindlimb lactate release by 50%. 5-HT decreased oxygen uptake by 15% but had no significant effect on glucose uptake or hindlimb lactate release. Microdialysis recovery of glucose and lactate was significantly elevated by NE and ANG II and decreased by 5-HT. The calculated interstitial concentration of glucose was increased by NE and ANG II but decreased by 5-HT. The interstitial concentration of lactate was decreased by NE and ANG II but increased by 5-HT. In all cases, nitroprusside reversed the effects of the vasoconstrictors. These data indicate that increased nutritive blood flow enhances the exchange of glucose and lactate by improving the supply of glucose to and the removal of lactate from the interstitium.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell metabolism
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Newman, JMB (Dr John Newman)
UTAS Author:Rattigan, S (Professor Stephen Rattigan)
UTAS Author:Clark, MG (Professor Michael Clark)
ID Code:24669
Year Published:2002
Web of Science® Times Cited:16
Deposited By:Biochemistry
Deposited On:2002-08-01
Last Modified:2003-04-11

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