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A possible alternative to 17 beta-estradiol in a viviparous lizard, Tiliqua nigrolutea


Edwards, A and Jones, SM and Davies, NW, A possible alternative to 17 beta-estradiol in a viviparous lizard, Tiliqua nigrolutea, General and Comparative Endocrinology, 129, (2) pp. 114-121. ISSN 0016-6480 (2002) [Refereed Article]

DOI: doi:10.1016/S0016-6480(02)00523-3


We have detected an unusual polar steroid as a major end product of gonadal steroid biosynthesis in male and female blotched blue-tongued lizards, Tiliqua nigrolutea. In an investigation of the steroidogenic pathways in blue-tongued lizards, we incubated gonads of both male and female lizards with tritiated pregnenolone and identified the products of steroid biosynthesis using thin layer chromatography and high performance liquid chromatography with radiometric detection: gonads from both reproductively active and quiescent lizards were examined. The proportion of total radioactive substrate converted to the unknown polar steroid was greater in individuals with hypertrophied gonads (males (N=4) 28.1%; females (N=4) 45.3%) than in those with regressed gonads (males (N=4) 5.9%; females (N=4) 33.3%). Properties of the unknown steroid were identical across all incubations. Incubation time-course information, thin layer and high performance liquid chromatography properties of the polar steroid are presented. The steroid is more polar than 17β-estradiol, which was not detected, but less polar than all tri-hydroxylated estrogens to which it was compared. The possibility of an alternative estrogen to 17β-estradiol in some reptiles is discussed.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Comparative physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Edwards, A (Dr Ashley Edwards)
UTAS Author:Jones, SM (Professor Susan Jones)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
ID Code:24664
Year Published:2002
Web of Science® Times Cited:9
Deposited By:Zoology
Deposited On:2002-08-01
Last Modified:2014-04-17

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