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Genetic dissection of the human leukocyte antigen region by use of haplotypes of Tasmanians with multiple sclerosis


Rubio, J and Bahlo, M and Butzkueven, H and Van der Mei, IAF and Sale, MM and Dickinson, JL and Groom, PS and Johnson, L and Simmons, R and Tait, B and Varney, M and Taylor, BVM and Dwyer, T and Williamson, R and Gough, N and Kilpatrick, T and Speed, T and Foote, SJ, Genetic dissection of the human leukocyte antigen region by use of haplotypes of Tasmanians with multiple sclerosis, American Journal of Human Genetics, 70, (5) pp. 1125-1137. ISSN 0002-9297 (2002) [Refereed Article]

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Copyright 2002 The American Society of Human Genetics

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DOI: doi:10.1086/339932


Association of multiple sclerosis (MS) with the human leukocyte antigen (HLA) class II haplotype DRB1*1501-DQB1*0602 is the most consistently replicated finding of genetic studies of the disease. However, the high level of linkage disequilibrium (LD) in the HLA region has hindered the identification of other loci that single-marker tests for association are unlikely to resolve. In order to address this issue, we generated haplotypes spanning 14.754 Mb (5 cM) across the entire HLA region. The haplotypes, which were inferred by genotyping relatives of 152 patients with MS and 105 unaffected control subjects of Tasmanian ancestry, define a genomic segment from D6S276 to D6S291, including 13 microsatellite markers integrated with allele-typing data for DRB1 and DQB1. Association to the DRB1*1501-DQB1*0602 haplotype was replicated. In addition, we found that the class I/extended class I region, defined by a genomic segment of бн400 kb between MOGCA and D6S265, harbors genes that independently increase risk of, or provide protection from, MS. Log-linear modeling analysis of constituent haplotypes that represent genomic regions containing class I (MOGCA-D6S265), class III (TNFa-TNFd-D6S273), and class II (DRB1-DQB1) genes indicated that having class I and class II susceptibility variants on the same haplotype provides an additive effect on risk. Moreover, we found no evidence for a disease locus in the class III region defined by a 150-kb genomic segment containing the TNF locus and 14 other genes. A global overview of LD performed using GOLD identified two discrete blocks of LD in the HLA region that correspond well with previous findings. We propose that the analysis of haplotypes, by use of the types of approaches outlined in the present article, should make it possible to more accurately define the contribution of the HLA to MS.

Item Details

Item Type:Refereed Article
Keywords:class-II region linkage disequilibrium, HLA-DR, genome screen, susceptibility, disease, recombination, polymorphism, association, DQ
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Medical genetics (excl. cancer genetics)
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Van der Mei, IAF (Professor Ingrid van der Mei)
UTAS Author:Sale, MM (Dr Michele Sale)
UTAS Author:Dickinson, JL (Professor Joanne Dickinson)
UTAS Author:Groom, PS (Sr Patricia Groom)
UTAS Author:Varney, M (Dr Michael Varney)
UTAS Author:Taylor, BVM (Professor Bruce Taylor)
UTAS Author:Dwyer, T (Professor Terry Dwyer)
UTAS Author:Foote, SJ (Professor Simon Foote)
ID Code:24648
Year Published:2002
Web of Science® Times Cited:78
Deposited By:Menzies Centre
Deposited On:2002-08-01
Last Modified:2013-03-06

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