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Phylogenetic analysis of the downy mildew pathogen of oilseed poppy in Tasmania, and its detection by PCR
Citation
Scott, JB and Hay, FS and Wilson, CR, Phylogenetic analysis of the downy mildew pathogen of oilseed poppy in Tasmania, and its detection by PCR, Mycological Research, 108 pp. 198-205. ISSN 0953-7562 (2004) [Refereed Article]
DOI: doi:10.1017/S095375620300916X
Abstract
Downy mildew of oilseed poppy (Papaver somniferum) has become a serious disease issue for the Tasmanian poppy industry since its first record in 1996. Previous reports have reported the pathogen as Peronospora arborescens, which is differentiated from the related species P. cristata, also known to infect Papaver spp., by conidium dimensions alone. This study investigated the taxonomic status of the downy mildew pathogen, using both morphological characters and molecular analysis of the internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA). The inherent variability of conidium dimensions made differentiation of species difficult. Sequence homology and phylogenetic analyses of the ITS region showed the pathogen to be more closely related to P. cristata than P. arborescens. It is therefore proposed that downy mildew of oilseed poppy in Tasmania be reattributed to the pathogen P. cristata. In addition to this work, PCR primers have been developed for the specific detection of the downy mildew pathogen in Tasmania.
Item Details
Item Type: | Refereed Article |
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Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Crop and pasture production |
Research Field: | Crop and pasture protection (incl. pests, diseases and weeds) |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Horticultural crops |
Objective Field: | Horticultural crops not elsewhere classified |
UTAS Author: | Scott, JB (Dr Jason Scott) |
UTAS Author: | Hay, FS (Dr Frank Hay) |
UTAS Author: | Wilson, CR (Professor Calum Wilson) |
ID Code: | 24440 |
Year Published: | 2004 |
Web of Science® Times Cited: | 19 |
Deposited By: | Agricultural Science |
Deposited On: | 2004-08-01 |
Last Modified: | 2011-11-22 |
Downloads: | 0 |
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