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Heterothallism among South Australian clonal lines of Uncinula necator


Evans, KJ and Scott, ES and Whisson, DL, Heterothallism among South Australian clonal lines of Uncinula necator, Australasian Plant Pathology, 26, (1) pp. 10-20. ISSN 0815-3191 (1997) [Refereed Article]

DOI: doi:10.1071/AP97002


The existence of two mating types and heterothallism among South Australian clonal lines of Uncinula necator was confirmed. An in vitro mating system for U. necator was established for identifying mating type and for future use in genetic studies. Cleistothecia, containing viable ascospores, formed on micropropagated grapevines in vitro following co-inoculation of two clonal lines of U, necator of opposite mating type. Specific incubation conditions were required, however, for the initiation of cleistothecia. These findings are discussed in relation to potential mechanisms regulating sexual reproduction. Among 20 clonal lines from South Australia tested, ten lines were identified as the 'plus' mating type and seven lines were identified as the 'minus' mating type. The mating type of the remaining three clonal lines has not been confirmed. Six of the 17 lines were paired in all possible combinations, and cleistothecia formed in all pairings where they were expected to occur for a heterothallic ascomycete with two mating types. Ascospores recovered from these controlled crosses produced typical colonies of U. necator when germinated on detached grapevine leaves.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Evans, KJ (Associate Professor Katherine Evans)
ID Code:24429
Year Published:1997
Web of Science® Times Cited:12
Deposited By:Agricultural Science
Deposited On:2002-08-01
Last Modified:2011-09-21

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