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Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5-14 years) cryostorage


Spurr, EE and Wiggins, NE and Marsden, KA and Lowenthal, RM and Ragg, SJ, Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5-14 years) cryostorage, Cryobiology, 44 pp. 210-217. ISSN 0011-2240 (2002) [Refereed Article]

DOI: doi:10.1016/S0011-2240(02)00027-5


Harvesting of stem cells during the early phases of treatment with no immediate intention to perform a stem cell transplant is becoming an increasingly common practice. Such "insurance" harvests are often stored for many years before being needed for transplant in a subsequent relapse. The effect of long-term cryostorage (5-14 years) on the viability and functional capacity of haematopoietic stem cells (HSCs) was investigated in 40 bone marrow and peripheral blood harvests using standard in vitro methods, the colony forming unit-granulocyte/macrophage (CFU-GM) assay and a single platform viable CD34+ cell absolute count by flow cytometry. Forty percent of harvests had CD34+ HSC counts of at least 0.7×106/kg bodyweight and 85% had CFU-GM counts of at least 1.0×105/kg bodyweight, these values representing our institutional minimum requirements for safe transplantation. Based on these results, it appears that HSC collections can remain adequate for safe transplantation after up to 14 years of cryostorage. However, as deterioration of HSC quality and viability may occur, some precautions may be warranted, namely harvesting higher than normal numbers of HSCs in collections intended for long-term storage and repeating in vitro assays on harvests after long-term storage prior to transplantation. © 2002 Elsevier Science (USA). All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Oncology and carcinogenesis
Research Field:Oncology and carcinogenesis not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Spurr, EE (Ms Elisabeth Spurr)
UTAS Author:Marsden, KA (Dr Katherine Marsden)
UTAS Author:Lowenthal, RM (Professor Ray Lowenthal)
UTAS Author:Ragg, SJ (Dr Scott Ragg)
ID Code:24387
Year Published:2002
Web of Science® Times Cited:59
Deposited By:Medicine
Deposited On:2002-08-01
Last Modified:2003-03-20

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