eCite Digital Repository

Development of a method for reproducing epizootic ulcerative syndrome using controlled doses of Aphanomyces invadans in species with different salinity requirements

Citation

Catap, ES and Munday, BL, Development of a method for reproducing epizootic ulcerative syndrome using controlled doses of Aphanomyces invadans in species with different salinity requirements, Aquaculture, 209, (1-4) pp. 35-47. ISSN 0044-8486 (2002) [Refereed Article]

DOI: doi:10.1016/S0044-8486(01)00805-5

Abstract

Lesions typical of epizootic ulcerative syndrome (EUS) were induced in three-spot gourami, Trichogaster trichopterus, and sand whiting, Sillago ciliata, injected intramuscularly with controlled doses of Aphanomyces invadans zoospores, the fungal pathogen associated with the disease. Both species of fish exhibited chronic granulomatous response and inflammatory cells, predominantly macrophages and lymphocytes, infiltrated the muscle and skin tissues, at days 6-8 post-inoculation of 65 to 85 spores/fish. Based on the comparative granuloma counts and percentage of cellular infiltration in a sampled lesion area using image analysis, it was shown that the three-spot gouramis mounted a more vigorous response than the sand whiting. It was also observed that lesions in three-spot gouramis exhibited early signs of resolution than those in sand whiting. However, fish mortality was greater in EUS-affected three-spot gourami than in EUS-affected sand whiting. With this technique, we were able to describe and compare the sequential histopathology of EUS lesions in a freshwater (three-spot gourami) and an estuarine (sand whiting) fish species. © 2002 Elsevier Science B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Munday, BL (Dr Barry Munday)
ID Code:24335
Year Published:2002
Web of Science® Times Cited:7
Deposited By:Health Sciences A
Deposited On:2002-08-01
Last Modified:2011-08-02
Downloads:0

Repository Staff Only: item control page