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Diversity of the coat protein-coding region among Ilarvirus isolates infecting hop in Australia


Crowle, DR and Pethybridge, SJ and Leggett, GW and Sherriff, LJ and Wilson, CR, Diversity of the coat protein-coding region among Ilarvirus isolates infecting hop in Australia, Plant Pathology, 52, (5) pp. 655-662. ISSN 0032-0862 (2003) [Refereed Article]

DOI: doi:10.1046/j.1365-3059.2003.00918.x


Coat protein (CP) sequences of 17 Ilarvirus isolates were obtained from hops at three farms in Tasmania, Australia. Phylogenetic analysis of these sequences and additional database sequences indicated several Apple mosaic virus (ApMV) isolate clusters distinct from Prunus necrotic ringspot virus (PNRSV): one containing isolates from apple; one containing a single isolate from almond; a third containing Australian hop isolates of the 'apple' serotype and a German isolate of unknown origin; and a fourth containing Australian hop isolates of the 'intermediate' serotype. Isolates from hop, pear and prune from the Czech Republic either formed a fifth grouping, or were divergent members of the 'intermediate' serotype group. Deduced amino acid (aa) residue differences between the coat proteins of the two hop isolate serotype groups were highlighted as possible regions of serological differentiation. No evidence for coinfection of plants with both serotypes was found. Tests of ApMV-infected hop buds using the Shirofugen flowering cherry assay revealed a possible differentiation of the two strains based on hypersensitivity. Because of serological similarities to PNRSV, these viruses have commonly been reported as strains of PNRSV. However, this study shows ilarviruses from Australian hops are strains of ApMV, but distinct from those infecting Malus spp.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Crowle, DR (Mr Damian Crowle)
UTAS Author:Pethybridge, SJ (Dr Sarah Pethybridge)
UTAS Author:Wilson, CR (Professor Calum Wilson)
ID Code:23727
Year Published:2003
Web of Science® Times Cited:16
Deposited By:Agricultural Science
Deposited On:2003-08-01
Last Modified:2004-04-21

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