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Anti-immunoglobulin binding and activation of snapper (Pagrus auratus) leucocytes

Citation

Morrison, RN and Hayball, JD and Cook, MT and Nowak, BF, Anti-immunoglobulin binding and activation of snapper (Pagrus auratus) leucocytes, Developmental & Comparative Immunology, 26, (3) pp. 247-255. ISSN 0145-305X (2002) [Refereed Article]

DOI: doi:10.1016/S0145-305X(01)00070-2

Abstract

In order to perform specific immunological assays we have produced and characterised three monoclonal antibodies (MAbs) that bind snapper (Pagrus auratus, Bloch and Schneider) immunoglobulin (Ig). Hybridomas were produced and screened for anti-Ig production using ELISA, Western blot and flow cytometry. All three MAbs (designated 2C5, 4A2 and 1C6) bound specifically to the heavy (H) chain of reduced Ig in Western blot. Furthermore, 1C6 was shown to bind to reduced skin mucus Ig H chain and all three MAbs cross-reacted with the H chain of Atlantic salmon and rainbow trout Ig. In flow cytometric analyses 2C5 and 4A2 bound to B cell populations in the peripheral blood and lymphoid organs. Furthermore, cross-linked 2C5 induced an increase in intracellular protein tyrosine phosphorylation in peripheral blood lymphocytes. Phosphorylated proteins exhibited similar molecular weights to those of mammalian Igα and Igβ and may represent snapper mIg accessory molecule analogues. These data exhibit the potential use of 2C5, 4A2 and 1C6 in both cellular and biochemical analyses of populations of snapper leucocytes. © 2002 Elsevier Science Ltd. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Morrison, RN (Dr Richard Morrison)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:23683
Year Published:2002
Web of Science® Times Cited:7
Deposited By:TAFI - Aquaculture
Deposited On:2002-08-01
Last Modified:2011-10-03
Downloads:0

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