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Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media

Citation

Wilson, TK and Carson, J, Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media, Letters in Applied Microbiology, 32, (5) pp. 326-330. ISSN 0266-8254 (2001) [Refereed Article]

DOI: doi:10.1046/j.1472-765X.2001.00906.x

Abstract

Aims: To create a fast, sensitive and inexpensive high-throughput method for the extraction of bacterial genomic DNA from selective-enrichment culture media. Methods and Results: Lysis of bacteria was achieved using guanidinium isothiocyanate, and DNA was extracted using 96-well glass microfibre filtration plates. Extraction-PCR detected the presence of 1 cfu Yersinia ruckeri and 16 cfu Lactococcus garvieae 200 μl-1 sample of selective-enrichment medium. Conclusions: An efficient method for high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media was achieved. Significance and Impact of the Study: This method enables detection of covert bacterial infections in fish. The simultaneous extraction of large numbers of samples allows for its use in bacterial monitoring programmes and quarantine.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Wilson, TK (Ms Teresa Wilson)
Author:Carson, J (Dr Jeremy Carson)
ID Code:23104
Year Published:2001
Web of Science® Times Cited:20
Deposited By:TAFI - Marine Research Laboratory
Deposited On:2001-08-01
Last Modified:2002-05-10
Downloads:0

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