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Identification of two down-regulated genes in rat liver allografts by mRNA differential display

journal contribution
posted on 2023-05-16, 13:13 authored by Lin, YC, Goto, S, Pan, TL, Hong, YR, Lin, CL, Lord, RJ, Chiang, KC, Lai, CY, Tseng, HP, Hsu, LW, Iwashita, S, Kitano, S, Chen, CL
Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models. DA (RT1a) donor livers were transplanted into DA, PVG (RT1c), and LEW (RT1l) recipients: (1) syngeneic OLT (DA-DA): no rejection occurs; (2) allogeneic OLT (DA-PVG): rejection occurs, but is naturally overcome without immuno-suppression; (3) allogeneic OLT (DA-LEW): animals die of acute rejection within 14 days. cDNA was isolated from selected bands, re-amplified for sequencing, and confirmed by Northern blots. Two down-regulated genes were observed in day-7 allogeneic OLT livers (DA-PVG, DA-LEW), while they were consistently expressed in day-7 syngeneic OLT (DA-DA) livers. These two genes were identified as α-glutathione sulfotransferase (α-GST) Ya gene and estrogen sulfotransferase (EST), respectively. Northern blots confirmed that their expression was down-regulated in OLT (DA-PVG) livers on days 7-26 and gradually restored. The mRNA expression of GST and EST may be good markers to predict rejection or induction of tolerance.

History

Publication title

Transplant International

Volume

14

Pagination

153-158

ISSN

0934-0874

Department/School

Tasmanian School of Medicine

Publisher

Springer-Verlag

Place of publication

USA

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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