File(s) not publicly available
Molecular characterization of the brassinosteroid-deficient lkb mutant in pea
journal contribution
posted on 2023-05-16, 13:12 authored by Schultz, L, Kerckhoffs, LH, Klahre, U, Yokota, T, James ReidJames ReidThe brassinosteriod-deficient lkb mutant of garden pea (Pistum sativum L.) is characterized by an erectoides phenotype (reduced internode length, thickened stems, epinastic leaves), which is rescued by application of exogenous brassinolide. We show that the LKB gene is the Arabidopsis DIMINUTO/DWARF-1 (DIM/DWF1) homologue of pea. The DIM/DWF1 homologue from lkb plants contains a mutation that may result in reduced enzyme function, thus resulting in the previously shown accumulation of 24-methylenecholesterol and a deficiency of its hydrogenated product, campesterol. This ultimately leads to a deficiency of the biologically active brassionolide. The mutation in the lkb sequence cosegregates with the lkb phenotype. Northern analyis of the LKB gene revealed that the gene is ubiquitously expressed around the plant and that there is no evidence for negative feedback regulation of the gene.
History
Publication title
Plant Molecular BiologyVolume
47Issue
4Pagination
491-498ISSN
0167-4412Department/School
School of Natural SciencesPublisher
Kluwer Academic PublPlace of publication
NetherlandsRepository Status
- Restricted