The brassinosteriod-deficient lkb mutant of garden pea (Pistum sativum L.) is characterized by an erectoides phenotype (reduced internode length, thickened stems, epinastic leaves), which is rescued by application of exogenous brassinolide. We show that the LKB gene is the Arabidopsis DIMINUTO/DWARF-1 (DIM/DWF1) homologue of pea. The DIM/DWF1 homologue from lkb plants contains a mutation that may result in reduced enzyme function, thus resulting in the previously shown accumulation of 24-methylenecholesterol and a deficiency of its hydrogenated product, campesterol. This ultimately leads to a deficiency of the biologically active brassionolide. The mutation in the lkb sequence cosegregates with the lkb phenotype. Northern analyis of the LKB gene revealed that the gene is ubiquitously expressed around the plant and that there is no evidence for negative feedback regulation of the gene.

Item Type:	Refereed Article
Research Division:	Biological Sciences
Research Group:	Genetics
Research Field:	Gene Expression (incl. Microarray and other genome-wide approaches)
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Environmental Sciences
UTAS Author:	Schultz, L (Dr Lee Schultz)
UTAS Author:	Kerckhoffs, LH (Dr Leonardus Kerckhoffs)
UTAS Author:	Reid, JB (Professor Jim Reid)
ID Code:	22822
Year Published:	2001
Web of Science® Times Cited:	34
Deposited By:	Plant Science
Deposited On:	2001-08-01
Last Modified:	2010-11-08
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