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Molecular characterization of the brassinosteroid-deficient lkb mutant in pea
Citation
Schultz, L and Kerckhoffs, LH and Klahre, U and Yokota, T and Reid, JB, Molecular characterization of the brassinosteroid-deficient lkb mutant in pea, Plant Molecular Biology, 47, (4) pp. 491-498. ISSN 0167-4412 (2001) [Refereed Article]
DOI: doi:10.1023/A:1011894812794
Abstract
The brassinosteriod-deficient lkb mutant of garden pea (Pistum sativum L.) is characterized by an erectoides phenotype (reduced internode length, thickened stems, epinastic leaves), which is rescued by application of exogenous brassinolide. We show that the LKB gene is the Arabidopsis DIMINUTO/DWARF-1 (DIM/DWF1) homologue of pea. The DIM/DWF1 homologue from lkb plants contains a mutation that may result in reduced enzyme function, thus resulting in the previously shown accumulation of 24-methylenecholesterol and a deficiency of its hydrogenated product, campesterol. This ultimately leads to a deficiency of the biologically active brassionolide. The mutation in the lkb sequence cosegregates with the lkb phenotype. Northern analyis of the LKB gene revealed that the gene is ubiquitously expressed around the plant and that there is no evidence for negative feedback regulation of the gene.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biological Sciences |
Research Group: | Genetics |
Research Field: | Gene expression (incl. microarray and other genome-wide approaches) |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the environmental sciences |
UTAS Author: | Schultz, L (Dr Lee Schultz) |
UTAS Author: | Kerckhoffs, LH (Dr Leonardus Kerckhoffs) |
UTAS Author: | Reid, JB (Professor Jim Reid) |
ID Code: | 22822 |
Year Published: | 2001 |
Web of Science® Times Cited: | 37 |
Deposited By: | Plant Science |
Deposited On: | 2001-08-01 |
Last Modified: | 2010-11-08 |
Downloads: | 0 |
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