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In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.)


Roy, AT and Leggett, G and Koutoulis, A, In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.), Plant Cell Reports, 20, (6) pp. 489-495. ISSN 0721-7714 (2001) [Refereed Article]

DOI: doi:10.1007/s002990100364


This paper describes an efficient colchicine-mediated technique for the in vitro induction of hop tetraploids and its confirmation by flow cytometry. A window of conditions generated a high percentage (>20%) of tetraploid induction, with the highest induction (25.6%) achieved with 0.05% colchicine for 48 h. Colchicine-induced tetraploids remained stable after 6 months in soil. Leaf characteristics of diploid and tetraploid hops were compared, and it was determined that stomatal length and width are suitable parameters for identifying putative hop tetraploids. As well as generating tetraploids, this technique generates mixoploid hops. Calli, derived from mixoploid leaves, were induced to form shoot buds and shoots. Individual shoots were classed as diploid, mixoploid or tetraploid after screening by flow cytometry. This callus-based technique can be employed when a genome-doubling agent generates mixoploids but fails to generate tetraploids.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genetics not elsewhere classified
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally sustainable plant production
Objective Field:Environmentally sustainable plant production not elsewhere classified
UTAS Author:Koutoulis, A (Professor Anthony Koutoulis)
ID Code:22808
Year Published:2001
Web of Science® Times Cited:83
Deposited By:Plant Science
Deposited On:2001-08-01
Last Modified:2011-08-03

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