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In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.)
journal contribution
posted on 2023-05-16, 13:11 authored by Roy, AT, Leggett, G, Anthony KoutoulisAnthony KoutoulisThis paper describes an efficient colchicine-mediated technique for the in vitro induction of hop tetraploids and its confirmation by flow cytometry. A window of conditions generated a high percentage (>20%) of tetraploid induction, with the highest induction (25.6%) achieved with 0.05% colchicine for 48 h. Colchicine-induced tetraploids remained stable after 6 months in soil. Leaf characteristics of diploid and tetraploid hops were compared, and it was determined that stomatal length and width are suitable parameters for identifying putative hop tetraploids. As well as generating tetraploids, this technique generates mixoploid hops. Calli, derived from mixoploid leaves, were induced to form shoot buds and shoots. Individual shoots were classed as diploid, mixoploid or tetraploid after screening by flow cytometry. This callus-based technique can be employed when a genome-doubling agent generates mixoploids but fails to generate tetraploids.
History
Publication title
Plant Cell ReportsVolume
20Issue
6Pagination
489-495ISSN
0721-7714Department/School
School of Natural SciencesPublisher
Springer-VerlagPlace of publication
New YorkRepository Status
- Restricted