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Migration of Langerhans cells and + dendritic cells from UV-B-irradiated sheep skin


Dandie, GW and Clydesdale, GJ and Radcliff, FJ and Muller, HK, Migration of Langerhans cells and aa+ dendritic cells from UV-B-irradiated sheep skin, Immunology and Cell Biology, 79, (1) pp. 41-48. ISSN 0818-9641 (2001) [Refereed Article]

DOI: doi:10.1046/j.1440-1711.2001.00975.x


Depletion of dendritic cells from UV-B-irradiated sheep skin was investigated by monitoring migration of these cells towards regional lymph nodes. By creating and cannulating pseudoafferent lymphatic vessels draining a defined region of skin, migrating cells were collected and enumerated throughout the response to UV-B irradiation. In the present study, the effects of exposing sheep flank skin to UV-B radiation clearly demonstrated a dose-dependent increase in the migration of Langerhans cells (LC) from the UV-B-exposed area to the draining lymph node. The range of UV-B doses assessed in this study included 2.7 kJ/m2, a suberythemal dose; 8 kJ/m2, 1 minimal erythemal dose (MED); 20.1 kJ/m2; 40.2 kJ/m2; and 80.4 kJ/m2, 10 MED. The LC were the cells most sensitive to UV-B treatment, with exposure to 8 kJ/m2 or greater reproducibly causing a significant increase in migration. Migration of γδ+ dendritic cells (γδ+ DC) from irradiated skin was also triggered by exposure to UV-B radiation, but dose dependency was not evident within the range of UV-B doses examined. This, in conjunction with the lack of any consistent correlation between either the timing or magnitude of migration peaks of these two cell types, suggests that different mechanisms govern the egress of LC and γδ+ DC from the skin. It is concluded that the depression of normal immune function in the skin after exposure to erythemal doses of UV-B radiation is associated with changes in the migration patterns of epidermal dendritic cells to local lymph nodes.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Dandie, GW (Dr Geoffrey Dandie)
UTAS Author:Clydesdale, GJ (Mr Gavin Clydesdale)
UTAS Author:Radcliff, FJ (Dr Jane Radcliff)
UTAS Author:Muller, HK (Professor Konrad Muller)
ID Code:22798
Year Published:2001
Web of Science® Times Cited:18
Deposited By:Pathology
Deposited On:2001-08-01
Last Modified:2002-05-23

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