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Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau
Citation
Watts, M and Munday, BL and Burke, CM, Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau, Fish & Shellfish Immunology, 11, (6) pp. 491-503. ISSN 1050-4648 (2001) [Refereed Article]
DOI: doi:10.1006/fsim.2000.0329
Abstract
Specific and total serum immunoglobulins were extracted by immunoaffinity, mannan-binding protein and Protein A affinity chromatography from southern bluefin tuna (Thunnus maccoyii Castelnau) immunised with rabbit IgG, and from non-immunised southern bluefin tuna. SDS-PAGE in 10% reducing gels revealed two heavy chains with molecular weights of approximately 74·6±1·3kDa and 71·2±0·9kDa, and two light chains with molecular weights of approximately 29±1·2kDa and 28±1·0kDa. Under non-reducing, but denaturing, conditions in 4% and 5% SDS-PAGE gels, a high molecular weight and a low molecular weight fraction were demonstrated. By gel filtration using Sephacryl HR300 a molecular weight of 845kDa, consistent with a tetramer, was obtained for the high molecular weight fraction, and a molecular weight of 168kDa, consistent with a monomer, was obtained for the low molecular weight fraction. The extinction coefficient at A280for the purified immunoglobulin (Ig) was determined to be 1·24. Tuna α-rabbit IgG Ig was reactive with all non-reduced mammalian IgG antigens tested, suggesting that common conformational antigenic determinants were recognised. © 2001 Academic Press.
Item Details
Item Type: | Refereed Article |
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Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Aquaculture |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Fisheries - aquaculture not elsewhere classified |
UTAS Author: | Watts, M (Dr Marianne Watts) |
UTAS Author: | Munday, BL (Dr Barry Munday) |
UTAS Author: | Burke, CM (Dr Chris Burke) |
ID Code: | 22583 |
Year Published: | 2001 |
Web of Science® Times Cited: | 35 |
Deposited By: | Health Sciences A |
Deposited On: | 2001-08-01 |
Last Modified: | 2011-10-04 |
Downloads: | 0 |
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