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Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau

Citation

Watts, M and Munday, BL and Burke, CM, Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau, Fish & Shellfish Immunology, 11, (6) pp. 491-503. ISSN 1050-4648 (2001) [Refereed Article]

DOI: doi:10.1006/fsim.2000.0329

Abstract

Specific and total serum immunoglobulins were extracted by immunoaffinity, mannan-binding protein and Protein A affinity chromatography from southern bluefin tuna (Thunnus maccoyii Castelnau) immunised with rabbit IgG, and from non-immunised southern bluefin tuna. SDS-PAGE in 10% reducing gels revealed two heavy chains with molecular weights of approximately 74·6±1·3kDa and 71·2±0·9kDa, and two light chains with molecular weights of approximately 29±1·2kDa and 28±1·0kDa. Under non-reducing, but denaturing, conditions in 4% and 5% SDS-PAGE gels, a high molecular weight and a low molecular weight fraction were demonstrated. By gel filtration using Sephacryl HR300 a molecular weight of 845kDa, consistent with a tetramer, was obtained for the high molecular weight fraction, and a molecular weight of 168kDa, consistent with a monomer, was obtained for the low molecular weight fraction. The extinction coefficient at A280for the purified immunoglobulin (Ig) was determined to be 1·24. Tuna α-rabbit IgG Ig was reactive with all non-reduced mammalian IgG antigens tested, suggesting that common conformational antigenic determinants were recognised. © 2001 Academic Press.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Watts, M (Dr Marianne Watts)
Author:Munday, BL (Dr Barry Munday)
Author:Burke, CM (Dr Chris Burke)
ID Code:22583
Year Published:2001
Web of Science® Times Cited:32
Deposited By:Health Sciences A
Deposited On:2001-08-01
Last Modified:2011-10-04
Downloads:0

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