Specific and total serum immunoglobulins were extracted by immunoaffinity, mannan-binding protein and Protein A affinity chromatography from southern bluefin tuna (Thunnus maccoyii Castelnau) immunised with rabbit IgG, and from non-immunised southern bluefin tuna. SDS-PAGE in 10% reducing gels revealed two heavy chains with molecular weights of approximately 74·6±1·3kDa and 71·2±0·9kDa, and two light chains with molecular weights of approximately 29±1·2kDa and 28±1·0kDa. Under non-reducing, but denaturing, conditions in 4% and 5% SDS-PAGE gels, a high molecular weight and a low molecular weight fraction were demonstrated. By gel filtration using Sephacryl HR300 a molecular weight of 845kDa, consistent with a tetramer, was obtained for the high molecular weight fraction, and a molecular weight of 168kDa, consistent with a monomer, was obtained for the low molecular weight fraction. The extinction coefficient at A280for the purified immunoglobulin (Ig) was determined to be 1·24. Tuna α-rabbit IgG Ig was reactive with all non-reduced mammalian IgG antigens tested, suggesting that common conformational antigenic determinants were recognised. © 2001 Academic Press.

Item Type:	Refereed Article
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Fisheries sciences
Research Field:	Aquaculture
Objective Division:	Animal Production and Animal Primary Products
Objective Group:	Fisheries - aquaculture
Objective Field:	Fisheries - aquaculture not elsewhere classified
UTAS Author:	Watts, M (Dr Marianne Watts)
UTAS Author:	Munday, BL (Dr Barry Munday)
UTAS Author:	Burke, CM (Dr Chris Burke)
ID Code:	22583
Year Published:	2001
Web of Science® Times Cited:	35
Deposited By:	Health Sciences A
Deposited On:	2001-08-01
Last Modified:	2011-10-04
Downloads:	0