The complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus (GGNNV), Singapore strain, were determined. 5′RACE and RNA ligation were used to obtain the complete nucleotide sequences of the 5′ and 3′ non-coding regions (NCRs). GGNNV RNA1 was determined to be 3103 nt long, containing an ORF of 982 aa, while GGNNV RNA2 was determined to be 1433 nt long, containing an ORF of 338 aa. Both GGNNV RNAs are longer than those of other published betanodavirus sequences and the additional nucleotides were located within the NCRs. Analysis of GGNNV RNA2 revealed that it is closely related to redspotted grouper nervous necrosis virus and that both grouper viruses share the same neutralization epitope. Predicted domains for six RNA-dependent RNA polymerase motifs and two putative ORFs (proteins B1 and B2) were confirmed by sequence analysis of GGNNV RNA1.

Item Type:	Refereed Article
Research Division:	Agricultural and Veterinary Sciences
Research Group:	Fisheries Sciences
Research Field:	Aquaculture
Objective Division:	Animal Production and Animal Primary Products
Objective Group:	Environmentally Sustainable Animal Production
Objective Field:	Environmentally Sustainable Animal Production not elsewhere classified
Author:	Munday, BL (Dr Barry Munday)
ID Code:	22550
Year Published:	2001
Web of Science® Times Cited:	81
Deposited By:	Health Sciences A
Deposited On:	2001-08-01
Last Modified:	2011-11-07
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