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Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain
Citation
Tan, C and Huang, B and Chang, SF and Ngoh, GH and Munday, BL and Chen, SC and Kwang, J, Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain, Journal of General Virology, 82 pp. 647-653. ISSN 0022-1317 (2001) [Refereed Article]
DOI: doi:10.1099/0022-1317-82-3-647
Abstract
The complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus (GGNNV), Singapore strain, were determined. 5′RACE and RNA ligation were used to obtain the complete nucleotide sequences of the 5′ and 3′ non-coding regions (NCRs). GGNNV RNA1 was determined to be 3103 nt long, containing an ORF of 982 aa, while GGNNV RNA2 was determined to be 1433 nt long, containing an ORF of 338 aa. Both GGNNV RNAs are longer than those of other published betanodavirus sequences and the additional nucleotides were located within the NCRs. Analysis of GGNNV RNA2 revealed that it is closely related to redspotted grouper nervous necrosis virus and that both grouper viruses share the same neutralization epitope. Predicted domains for six RNA-dependent RNA polymerase motifs and two putative ORFs (proteins B1 and B2) were confirmed by sequence analysis of GGNNV RNA1.
Item Details
Item Type: | Refereed Article |
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Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Aquaculture |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Environmentally sustainable animal production |
Objective Field: | Environmentally sustainable animal production not elsewhere classified |
UTAS Author: | Munday, BL (Dr Barry Munday) |
ID Code: | 22550 |
Year Published: | 2001 |
Web of Science® Times Cited: | 104 |
Deposited By: | Health Sciences A |
Deposited On: | 2001-08-01 |
Last Modified: | 2011-11-07 |
Downloads: | 0 |
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