eCite Digital Repository

Detection of nodavirus in barramundi, Lates calcarifer (Bloch), using recombinant coat protein-based ELISA and RT-PCR

Citation

Huang, B and Tan, C and Chang, SF and Munday, BL and Mathew, JA and Ngoh, GH and Kwang, J, Detection of nodavirus in barramundi, Lates calcarifer (Bloch), using recombinant coat protein-based ELISA and RT-PCR, Journal of Fish Diseases, 24, (3) pp. 135-141. ISSN 0140-7775 (2001) [Refereed Article]

DOI: doi:10.1046/j.1365-2761.2001.00270.x

Abstract

The coat protein encoded by the nodavirus RNA2 gene originally isolated from greasy grouper, Epinephelus tauvina, was cloned, expressed as a recombinant polyhistidine-tailed fusion protein and characterized by immunoblot analysis. The purified recombinant protein was used to develop an indirect enzyme-linked immunosorbent assay (ELISA) to detect body exudate and plasma antibodies against the coat protein in both experimentally infected and commercial barramundi. In addition, the nucleotide sequence was employed to develop a RT-PCR detection assay based on the T4 region. The results showed that the virus could be detected as early as 3 days post-infection by RT-PCR while antibodies against the recombinant coat protein were detectable on day 6 post-infection. Among 112 commercial barramundi samples collected from October 1999 to April 2000, 9% showed positive ELISA results which were further verified by Western blot.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Environmentally Sustainable Animal Production
Objective Field:Environmentally Sustainable Animal Production not elsewhere classified
Author:Munday, BL (Dr Barry Munday)
ID Code:22546
Year Published:2001
Web of Science® Times Cited:25
Deposited By:Health Sciences A
Deposited On:2001-08-01
Last Modified:2011-11-07
Downloads:0

Repository Staff Only: item control page