eCite Digital Repository

Identification of hydrolysable tannins in the reaction zone of Eucalyptus nitens wood by high performance liquid chromatography-electrospray ionisation mass spectrometry

Citation

Barry, KM and Davies, NW and Mohammed, CL, Identification of hydrolysable tannins in the reaction zone of Eucalyptus nitens wood by high performance liquid chromatography-electrospray ionisation mass spectrometry, Phytochemical Analysis, 12, (2) pp. 120-127. ISSN 0958-0344 (2001) [Refereed Article]

DOI: doi:10.1002/pca.548

Abstract

The first detailed analysis of the phenolic constituents of the reaction zones (tissue of antimicrobial defense) from the sapwood of a Eucalyptus spp. is presented. Plantation-grown Eucalyptus nitens trees with stem decay resulting from pruning wounds were sampled and extracts were prepared from healthy sapwood and from reaction zone tissue. Analysis by HPLC with ESI-MS revealed that a diverse range of hydrolysable tannins are present in both healthy sapwood and in reaction zone extracts, including over 30 gallotannins, ellagitannins and phenols. Eight tannins were unequivocally identified, including the gallotannins tri-O-galloyl-β-D-glucose, tetra-O-galloyl-β-D-glucose and penta-O-galloyl-β-D-glucose, and the ellagitannins pedunculagin, tellimagrandin I, casuarinin, casuarictin and tellimagrandin II. The phenols gallic acid, ellagic acid and catechin were also identified. The ellagitannins (particularly pedunculagin) are considerably more abundant in the reaction zone than in the healthy sapwood and may contribute to the effectiveness of the reaction zone as an antimicrobial barrier. Copyright © 2001 John Wiley & Sons, Ltd.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Forestry Sciences
Research Field:Forestry Pests, Health and Diseases
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood Plantations
Author:Barry, KM (Dr Karen Barry)
Author:Davies, NW (Associate Professor Noel Davies)
Author:Mohammed, CL (Professor Caroline Mohammed)
ID Code:22529
Year Published:2001
Web of Science® Times Cited:54
Deposited By:Central Science Laboratory
Deposited On:2001-08-01
Last Modified:2011-08-03
Downloads:0

Repository Staff Only: item control page