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Indirect Photometric Detection in CE Using Buffered Electrolytes - Part 1, Principles
Citation
Macka, M and Johns, CA and Doble, PA and Haddad, PR, Indirect Photometric Detection in CE Using Buffered Electrolytes - Part 1, Principles, LC GC North America, 19, (1) pp. 38-47. ISSN 1527-5949 (2001) [Refereed Article]
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Copyright Statement
Copyright © 2001 Advanstar Communications Inc.
Official URL: http://chromatographyonline.com
Abstract
Indirect photometric detection in capillary electrophoresis is valuable as a detection method for nonabsorbing low molecular weight ions. The general operating principle rests in the displacement of an absorbing ion — sometimes called the probe — added to the electrolyte by the analyte co-ions, which are ions of the same charge polarity, as they migrate under the influence of the electric field through the capillary. Despite this apparent simplicity, the choice of optimal parameters, and especially of electrolyte composition, can be quite challenging. This first installment of a two-part series outlines the principles and approaches to indirect detection.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| UTAS Author: | Macka, M (Professor Mirek Macka) |
| UTAS Author: | Johns, CA (Dr Cameron Johns) |
| UTAS Author: | Doble, PA (Mr Philip Andrew Doble) |
| UTAS Author: | Haddad, PR (Professor Paul Haddad) |
| ID Code: | 22237 |
| Year Published: | 2001 |
| Web of Science® Times Cited: | 40 |
| Deposited By: | Chemistry |
| Deposited On: | 2001-08-01 |
| Last Modified: | 2010-02-08 |
| Downloads: | 478 View Download Statistics |
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