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Indirect Photometric Detection in CE Using Buffered Electrolytes - Part 1, Principles


Macka, M and Johns, CA and Doble, PA and Haddad, PR, Indirect Photometric Detection in CE Using Buffered Electrolytes - Part 1, Principles, LC GC North America, 19, (1) pp. 38-47. ISSN 1527-5949 (2001) [Refereed Article]


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Copyright 2001 Advanstar Communications Inc.

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Indirect photometric detection in capillary electrophoresis is valuable as a detection method for nonabsorbing low molecular weight ions. The general operating principle rests in the displacement of an absorbing ion sometimes called the probe added to the electrolyte by the analyte co-ions, which are ions of the same charge polarity, as they migrate under the influence of the electric field through the capillary. Despite this apparent simplicity, the choice of optimal parameters, and especially of electrolyte composition, can be quite challenging. This first installment of a two-part series outlines the principles and approaches to indirect detection.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Johns, CA (Dr Cameron Johns)
UTAS Author:Doble, PA (Mr Philip Andrew Doble)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:22237
Year Published:2001
Web of Science® Times Cited:41
Deposited By:Chemistry
Deposited On:2001-08-01
Last Modified:2010-02-08
Downloads:509 View Download Statistics

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