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Modelling of migration behaviour of inorganic anions in ion-exchange capillary electrochromatography


Breadmore, MC and Hilder, EF and Macka, M and Avdalovic, N and Haddad, PR, Modelling of migration behaviour of inorganic anions in ion-exchange capillary electrochromatography, Electrophoresis, 22, (3) pp. 503-510. ISSN 0173-0835 (2001) [Refereed Article]

DOI: doi:10.1002/1522-2683(200102)22:3<503::AID-ELPS503>3.0.CO;2-S


A theoretical model to explain the observed mobility of inorganic anions in capillary electrochromatography (CEC) using ion-exchange (IE) stationary phases has been derived. The model divides contributions to the observed mobility of an analyte ion into capillary electrophoretic (CE) and IE components. The CE component includes the influence of varying the ionic strength of the background electrolyte on the electrophoretic mobility of the analyte, while the IE component accounts for the variation in retention of the analyte ion caused by changing the composition of the background electrolyte. The model was verified using a mixture of UV-absorbing inorganic ions in electrolytes of differing eluotropic strength in both packed and open-tubular CEC systems, with excellent agreement (r2>0.98) for both systems. Values of constants in the model equation determined by nonlinear regression were used to estimate the relative strengths of the interactions of different analytes with the stationary phase and these were found to agree well with elution orders observed in conventional IE chromatography.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Hilder, EF (Professor Emily Hilder)
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:22186
Year Published:2001
Web of Science® Times Cited:13
Deposited By:Chemistry
Deposited On:2001-08-01
Last Modified:2011-08-03

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