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Identification of Orchid fleck virus by Reverse Transcriptase-Polymerase Chain Reaction and Analysis of Isolate Relationships

Citation

Blanchfield, AL and Mackenzie, AM and Gibbs, A and Kondo, H and Tamada, T and Wilson, CR, Identification of Orchid fleck virus by Reverse Transcriptase-Polymerase Chain Reaction and Analysis of Isolate Relationships, Journal of Phytopathology, 149, (11-12) pp. 713-718. ISSN 0931-1785 (2001) [Refereed Article]

DOI: doi:10.1046/j.1439-0434.2001.00702.x

Abstract

Using reverse transcription-polymerase chain reaction (RT-PCR) tests with a primer complementary to a region of its nucleoprotein gene together with a polydT/SP6 primer, Orchid fleck virus (OFV) was detected in 34 samples of infected orchids of several different genera from world-wide sources. The resulting DNA fragments were approximately 800 bp in length and their sequences were determined directly. Analysis of sequences of the major open reading frame (ORF) within the DNA fragments obtained showed the presence of two virus strains. The first group was of two isolates, the original Japanese isolate from which the primers were derived and one from Germany, and the second group contained 33 isolates from four continents. The sequences of different groups differed from one another by at least 15.6% (nucleotide) and 1.8% (amino-acid), but the within-group differences were much less (<1.7% difference). A search of the international nucleotide database with the OFV sequences showed them to be related, but distantly, to nucleoprotein regions of the genomes of four plant-infecting rhabdoviruses.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Horticultural Crop Protection (Pests, Diseases and Weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Ornamentals, Natives, Flowers and Nursery Plants
Author:Blanchfield, AL (Miss Annette Blanchfield)
Author:Wilson, CR (Associate Professor Calum Wilson)
ID Code:22102
Year Published:2001
Web of Science® Times Cited:24
Deposited By:Agricultural Science
Deposited On:2001-08-01
Last Modified:2002-05-14
Downloads:0

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