eCite Digital Repository
Size-Dependent Effects of Microspheres on Vasoconstrictor-Mediated Change in Oxygen Uptake by Perfused Rat Hindlimb
Citation
Keske, MAV and Rattigan, S and Clark, MG, Size-Dependent Effects of Microspheres on Vasoconstrictor-Mediated Change in Oxygen Uptake by Perfused Rat Hindlimb, Microvascular Research, 62, (3) pp. 306-314. ISSN 0026-2862 (2001) [Refereed Article]
DOI: doi:10.1006/mvre.2001.2344
Abstract
There are two vascular flow routes in skeletal muscle that can be accessed by different vasoconstrictors acting at selective sites in the vascular tree. Thus, angiotensin II (AII) and serotonin (5-HT), which stimulate and inhibit metabolism, do so by directing flow to nutritive and non-nutritive routes, respectively. In the present study the association between vascular flow route recruitment and metabolism was assessed by embolism with microspheres of different sizes. Latex microspheres (MS) of four sizes, 5.4 (MS5), 11.8 (MS12), 23.4 (MS23), and 93.6 μm (MS94), were injected during AII- or 5-HT-mediated constriction or under basal conditions and the effects on hindlimb oxygen uptake (VO2), perfusion pressure, and venous flow rate were determined. MS5 or MS12 partially reversed 5-HT-mediated inhibition of VO2 by 39 and 55%, respectively (P < 0.05), fully reversed AII-mediated stimulation of VO2 (P < 0.05), stimulated basal VO2 (P < 0.05), and increased pressure while only marginally (< 10%) decreasing venous flow. MS23 or MS94 dose-dependently increased pressure and inhibited VO2, during basal or 5HT- and AII-mediated constriction, while only marginally decreasing venous flow. In conclusion, microspheres of less than 12 μm when injected into the constant flow perfused rat hindlimb can alter metabolism by altering flow distribution between nutritive and nonnutritive routes. Larger MS (≥24 μm) are nondiscriminating possibly because they exceed the size of vessels in which branch points to the two vascular routes are located. Overall the findings provide further evidence for two microvascular routes in muscle, one nutritive and the other nonnutritive. © 2001 Academic Press.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Biological Sciences |
Research Group: | Zoology |
Research Field: | Animal physiology - systems |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Keske, MAV (Dr Michelle Keske) |
UTAS Author: | Rattigan, S (Professor Stephen Rattigan) |
UTAS Author: | Clark, MG (Professor Michael Clark) |
ID Code: | 21979 |
Year Published: | 2001 |
Web of Science® Times Cited: | 2 |
Deposited By: | Biochemistry |
Deposited On: | 2001-08-01 |
Last Modified: | 2011-11-21 |
Downloads: | 0 |
Repository Staff Only: item control page