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Spatial Distribution of Nutritive and Nonnutritive Vascular Routes in Perfused Rat Hindlimb Muscle Using Microspheres

Citation

Keske, MAV and Rattigan, S and Clark, MG and Bernard, SL and Glenny, RW, Spatial Distribution of Nutritive and Nonnutritive Vascular Routes in Perfused Rat Hindlimb Muscle Using Microspheres, Microvascular Research, 61, (1) pp. 111-121. ISSN 0026-2862 (2001) [Refereed Article]

DOI: doi:10.1006/mvre.2000.2278

Abstract

Skeletal muscle appears to have two vascular flow routes, nutritive and nonnutritive, where the balance of flow is controlled by vasoconstrictors. In the present study, spatial distributions of the two flow routes in muscles of the perfused rat hindlimb were attempted using fluorescent microspheres (15 μm in diameter). Microspheres were injected during steady-state perfusion with norepinephrine (proposed recruiter of nutritive flow), serotonin (proposed recruiter of nonnutritive flow), or vehicle. The three-dimensional location of individual microspheres in representative muscles was determined using a Fluorescent Imaging CryoMicrotome. Norepinephrine and serotonin each increased perfusion pressure (P < 0.05) but stimulated and inhibited oxygen consumption (P < 0.05), respectively. The distribution of microspheres lodged in muscle was independent of the agent used. Spatial perfusion indices for norepinephrine, serotonin, and vehicle did not differ from each other. Similarly, there was no difference in these indices for a theoretical distribution where microspheres were deliberately positioned in muscle bundle capillaries or interfibrillar connective tissue vessels. We conclude that the nutritive and nonnutritive flow routes are distributed throughout muscle sections consistent with their locations in muscle bundle capillaries and interfibrillar connective tissue, respectively. © 2000 Academic Press.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Physiology
Research Field:Animal Physiology - Systems
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cardiovascular System and Diseases
Author:Keske, MAV (Dr Michelle Keske)
Author:Rattigan, S (Professor Stephen Rattigan)
Author:Clark, MG (Professor Michael Clark)
ID Code:21975
Year Published:2001
Web of Science® Times Cited:3
Deposited By:Biochemistry
Deposited On:2001-08-01
Last Modified:2011-11-21
Downloads:0

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