eCite Digital Repository
The usefulness of fatty acid analysis in differentiating species of Pythium, Rhizoctonia and Gaeumannomyces and as a tool for their detection in infected wheat roots
Citation
Pankhurst, CE and Pederson, HG and Hawke, BG, The usefulness of fatty acid analysis in differentiating species of Pythium, Rhizoctonia and Gaeumannomyces and as a tool for their detection in infected wheat roots, Australasian Plant Pathology, 30, (3) pp. 191-197. ISSN 0815-3191 (2001) [Refereed Article]
DOI: doi:10.1071/AP01018
Abstract
Principal component analysis (PCA) of cellular fatty acids extracted from cultures of Gaeumannomyces spp., Rhizoctonia solani (anastamosis groups AG4 and AG8) and Pythium spp. showed strong clustering of cultures within genera and good discrimination between genera. PCA of the fatty acid profiles was successful in distinguishing between seven different Pythium spp. tested (P. arrhenomannes, P. australe, P. echinulatum, P. irregulaṙe, P. ostracodes, P. spinosum and P. ultimum) but did not distinguish between the Gaeumannomyces spp. (G. graminis var. avenae, G. graminis var. graminis, G. graminis var. tritici, G. cylindrosporus and G. incrustans) nor the isolates of the two AG groups of Rhizoctonia examined. Significant differences between species were detected in the relative amounts of individual fatty acids. The presence of two fatty acids (20:4ω6c and 20:5ω3c), found only in the Pythium species examined, was used to detect the presence of P. echinulatum and P. irregulare in wheat roots infected with these two fungi.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Biological Sciences |
Research Group: | Plant Biology |
Research Field: | Plant Pathology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Winter Grains and Oilseeds |
Objective Field: | Wheat |
UTAS Author: | Pederson, HG (Dr Hugh Pederson) |
ID Code: | 21921 |
Year Published: | 2001 |
Web of Science® Times Cited: | 2 |
Deposited By: | Zoology |
Deposited On: | 2001-08-01 |
Last Modified: | 2011-08-03 |
Downloads: | 0 |
Repository Staff Only: item control page