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The usefulness of competitive PCR: airway gene expression of IL-5, IL-4, IL-4[delta]2, IL-2, and IFN[gamma] in asthma. (Statistical Data Included)
Citation
Glare, EM and Divjak, M and Bailey, MJ and Walters, EH, The usefulness of competitive PCR: airway gene expression of IL-5, IL-4, IL-4[delta]2, IL-2, and IFN[gamma] in asthma. (Statistical Data Included), Thorax, 56, (7) pp. 541-548. ISSN 0040-6376 (2001) [Refereed Article]
DOI: doi:10.1136/thorax.56.7.541
Abstract
Background - Asthma has been described as an eosinophilic bronchitis driven by interleukin(IL)-4 and IL-5. The quantification of cytokine mRNA levels in airway samples has been confounded by house-keeping gene expression which differs between and within asthmatics and controls. Methods - The usefulness of competitive reverse transcriptase-polymerase chain reaction (RT-PCR) that is independent of housekeeping gene expression for quantitating the mRNA for interferon (IFN)γ, IL-2, IL-5, IL-4 and its receptor antagonist encoding splicing variant IL-4δ2 was determined in a cross sectional study of 45 normal control subjects and 111 with asthma. Results - Atopic controls and atopic asthmatic subjects expressed more IL-5 than non-atopic controls (p<0.02) in bronchoalveolar lavage (BAL) cells, but not in biopsy specimens. IL-5 mRNA expression in BAL cells from asthmatic subjects using inhaled corticosteroids (ICS) was significantly lower than those not receiving ICS (p=0.04). IL-2 mRNA levels differed with steroid use in biopsy specimens but not in BAL cells. IFNγ, IL-4, and IL-4δ2 mRNA levels did not differ between any groups and were not affected by steroid use. IL-4 and IL-4δ2 mRNA levels were positively correlated (p<0.0001), suggesting coordinated transcription. Conclusions - While the signal differentiation of competitive PCR in asthma may rival that of in situ hybridisation and immunohistochemistry, the method is expensive and wasteful of material.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Cardiovascular medicine and haematology |
Research Field: | Respiratory diseases |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Walters, EH (Professor Haydn Walters) |
ID Code: | 21825 |
Year Published: | 2001 |
Web of Science® Times Cited: | 13 |
Deposited By: | Medicine |
Deposited On: | 2001-08-01 |
Last Modified: | 2002-07-04 |
Downloads: | 0 |
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