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The efficacy of a commercial beta-glucan preparation, EcoActiva (TM), on stimulating respiratory burst activity of head-kidney macrophages from pink snapper (Pagrus auratus), Sparidae

Citation

Cook, MT and Hayball, PJ and Hutchinson, W and Nowak, BF and Hayball, JD, The efficacy of a commercial beta-glucan preparation, EcoActiva (TM), on stimulating respiratory burst activity of head-kidney macrophages from pink snapper (Pagrus auratus), Sparidae, Fish & Shellfish Immunology, 11, (8) pp. 661-672. ISSN 1050-4648 (2001) [Refereed Article]

DOI: doi:10.1006/fsim.2001.0343

Abstract

This study investigated the in vitro effects of a commercial β-glucan preparation, EcoActiva™, on the respiratory burst activity of head-kidney macrophages isolated from pink snapper (Pagrus auratus), a marine fish cultured in Australia. Macrophages incubated with EcoActiva™ displayed morphological characteristics of activation, and were stimulated to produce superoxide, Pre-incubation with low levels of EcoActiva™ significantly increased the response to phorbol myristate acetate (PMA) and lipopolysaccharide (LPS), indicating that EcoActiVa™ could prime these macrophages. Co-culturing macrophages with both LPS and PMA, or EcoActiva™ and PMA, increased burst activity compared with the response to PMA alone, however, this increase was additive and not synergistic. These results suggest that EcoActiva™ is able to stimulate non-specific immunity in snapper through increased respiratory burst activity of macrophages, an important component of the host defence network. © 2001 Academic Press.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:21346
Year Published:2001
Web of Science® Times Cited:46
Deposited By:TAFI - Aquaculture
Deposited On:2001-08-01
Last Modified:2011-10-04
Downloads:0

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