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A screen of mammalian antibodies on snapper (Pagrus auratus, Sparidae) peripheral blood leukocytes reveals cross reactivity of an anti-human CD3 antibody with a population of mIg- cells

Citation

Cook, MT and Morrison, RN and Wilkinson, R and Nowak, BF and Hayball, PJ and Hayball, JD, A screen of mammalian antibodies on snapper (Pagrus auratus, Sparidae) peripheral blood leukocytes reveals cross reactivity of an anti-human CD3 antibody with a population of mIg- cells, Developmental & Comparative Immunology, 25, (7) pp. 553-559. ISSN 0145-305X (2001) [Refereed Article]

DOI: doi:10.1016/S0145-305X(01)00024-6

Abstract

Detailed immunological studies of the teleosts have been hampered by a lack of antibodies against cell-specific markers. Furthermore, where antibodies have been raised, in many instances they have been found to be species-specific. In comparison, many monoclonal and polyclonal antibodies exist with specificities for mammalian proteins and glycoproteins that effectively differentiate leukocyte sub-populations. In this study, we have tested a panel of 54 commercial antibodies against human and murine cell surface receptors for their ability to bind leukocytes isolated from the peripheral blood of snapper (Pagrus auratus). From this panel, one antibody, A452, which is specific for the intracytoplasmic tail of the epsilon (ε) chain of the T cell receptor-associated CD3 complex (CD3ε) bound to a subpopulation of peripheral blood leukocytes. Mutually exclusive counterstaining was observed when this antibody was used in conjunction with a monoclonal anti-snapper immunoglobulin antibody. This suggests that A452 may be binding to putative snapper T cells. Copyright © 2001 Elsevier Science Ltd.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Veterinary Sciences
Research Field:Veterinary Immunology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Morrison, RN (Dr Richard Morrison)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:21227
Year Published:2001
Web of Science® Times Cited:14
Deposited By:TAFI - Aquaculture
Deposited On:2001-08-01
Last Modified:2011-10-03
Downloads:0

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