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Development and validation of a new dot blot test for the detection of Paramoeba pemaquidensis (Page) in fish

Citation

Douglas-Helders, GM and Carson, J and Howard, T and Nowak, BF, Development and validation of a new dot blot test for the detection of Paramoeba pemaquidensis (Page) in fish, Journal of Fish Diseases, 24, (5) pp. 273-280. ISSN 0140-7775 (2001) [Refereed Article]

DOI: doi:10.1046/j.1365-2761.2001.00295.x

Abstract

In this study, the development of a dot blot assay to assess amoebic gill disease (AGD) using non-lethal gill mucus samples is described and its performance validated by comparing the assay with the indirect fluorescent antibody test (IFAT), the 'gold standard' test. The agreement between the two tests was high, with a positive predictive value of 95% and negative predictive value of 93%, with a corrected κ value of 0.88. The sensitivity and specificity of the test were 97 and 91%, respectively. The dot blot is both sensitive and specific for Paramoeba pemaquidensis and is formatted so that large numbers of samples can be conveniently analysed.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Douglas-Helders, GM (Dr Greetje Douglas-Helders)
Author:Carson, J (Dr Jeremy Carson)
Author:Howard, T (Dr Howard)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:21224
Year Published:2001
Web of Science® Times Cited:26
Deposited By:TAFI - Aquaculture
Deposited On:2001-08-01
Last Modified:2011-11-07
Downloads:0

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