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Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene Tg737, are required for assembly of cilia and flagella

Citation

Pazour, GJ and Dickert, BL and Vucica, Y and Scott Seeley, E and Rosenbaum, JL and Witman, GB and Cole, DG, Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene Tg737, are required for assembly of cilia and flagella, The Journal of Cell Biology, 151, (3) pp. 709-718. ISSN 0021-9525 (2000) [Refereed Article]

DOI: doi:10.1083/jcb.151.3.709

Abstract

Intraflagellar transport (IFT) is a rapid movement of multi-subunit protein particles along flagellar microtubules and is required for assembly and maintenance of eukaryotic flagella. We cloned and sequenced a Chlamydomonas cDNA encoding the IFT88 subunit of the IFT particle and identified a Chlamydomonas insertional mutant that is missing this gene. The phenotype of this mutant is normal except for the complete absence of flagella. IFT88 is homologous to mouse and human genes called Tg737. Mice with defects in Tg737 die shortly after the birth from polycystic kidney disease. We show that the primary cilia in the kidney of Tg737 mutant mice are shorter than normal. This indicates that IFT is important for primary cilia assembly in mammals. It is likely that primary cilia have an important function in the kidney and that defects in their assembly can lead to polycystic kidney disease.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene Expression (incl. Microarray and other genome-wide approaches)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Urogenital System and Disorders
Author:Vucica, Y (Ms Yvonne Vucica)
ID Code:20568
Year Published:2000
Web of Science® Times Cited:638
Deposited By:Plant Science
Deposited On:2000-08-01
Last Modified:2011-08-04
Downloads:0

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