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Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene Tg737, are required for assembly of cilia and flagella
journal contribution
posted on 2023-05-16, 12:38 authored by Pazour, GJ, Dickert, BL, Vucica, Y, Scott Seeley, E, Rosenbaum, JL, Witman, GB, Cole, DGIntraflagellar transport (IFT) is a rapid movement of multi-subunit protein particles along flagellar microtubules and is required for assembly and maintenance of eukaryotic flagella. We cloned and sequenced a Chlamydomonas cDNA encoding the IFT88 subunit of the IFT particle and identified a Chlamydomonas insertional mutant that is missing this gene. The phenotype of this mutant is normal except for the complete absence of flagella. IFT88 is homologous to mouse and human genes called Tg737. Mice with defects in Tg737 die shortly after the birth from polycystic kidney disease. We show that the primary cilia in the kidney of Tg737 mutant mice are shorter than normal. This indicates that IFT is important for primary cilia assembly in mammals. It is likely that primary cilia have an important function in the kidney and that defects in their assembly can lead to polycystic kidney disease.
History
Publication title
The Journal of Cell BiologyVolume
151Pagination
709-718ISSN
0021-9525Department/School
School of Natural SciencesPublisher
Rockefeller Univ PressPlace of publication
1114 First Ave, 4Th Fl, New York, USA, Ny, 10021Repository Status
- Restricted