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Sequence of Cellular Changes Following Localized Axotomy to Cortical Neurons in Glia-Free Culture


Dickson, TC and Adlard, PA and Vickers, JC, Sequence of Cellular Changes Following Localized Axotomy to Cortical Neurons in Glia-Free Culture, Journal of Neurotrauma, 17, (11) pp. 1095-1103. ISSN 0897-7151 (2000) [Refereed Article]

DOI: doi:10.1089/neu.2000.17.1095


This study utilizes an in vitro model of localized physical injury to axons to examine the specific responses of neocortical neurons to trauma in isolation from glia cell types. The neuronal response to axotomy was closely linked with nerve cell maturity. Cultures grown for 14 days in vitro showed no accumulation of either neurofilaments or, the axonal sprouting marker, GAP43, within injured axons following injury. In older cultures (21 days in vitro), however, temporally distinct axonal changes were evident following transection of axonal bundles. At 12 h postinjury, these included extensive accumulation of neurofilaments into ring-like structures within the cut stumps and an increase in puncture GAP43 labelling throughout the damaged area. At 24 h postinjury, bulb-like accumulations of neurofilaments were also present within the transected axons. Finally at 3 days postinjury, distinct GAP43 and neurofilament immunolabeled axons, and GAP43 immunopositive growth cones, emanated from the cut stump. These results indicate that injured axons of mature neurons undergo a defined series of reactive changes, ultimately culminating in a sprouting response, which occur independently of the presence or effects of glial cell populations.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Dickson, TC (Professor Tracey Dickson)
UTAS Author:Adlard, PA (Dr Paul Anthony Adlard)
UTAS Author:Vickers, JC (Professor James Vickers)
ID Code:20355
Year Published:2000
Web of Science® Times Cited:33
Deposited By:Pathology
Deposited On:2000-08-01
Last Modified:2001-06-12

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