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Analysing Cell Division in vivo Using Flow Cytometric Measurement of CFSE Dye Dilution


Lyons, AB, Analysing Cell Division in vivo Using Flow Cytometric Measurement of CFSE Dye Dilution, Journal of Immunological Methods, 243, (1-2) pp. 147-154. ISSN 0022-1759 (2000) [Refereed Article]

DOI: doi:10.1016/S0022-1759(00)00231-3


Since its introduction in 1994 (J. Immunol. Methods 171 (1994) 131), the flow cytometric analysis of lymphocyte proliferation by serial halving of the fluorescence intensity of the vital dye CFSE (carboxyfluorescein diacetate, succinimidyl ester or CFDA-SE) has become widely used in immunological laboratories around the world. This technique allows the visualisation of eight to 10 discrete cycles of cell division by flow cytometry, both in vitro and in vivo. Appropriately conjugated antibodies can be used to probe surface marker changes as cells divide, or changes in expression of internal molecules such as cytokines when appropriate fixation and permeabilisation protocols are used. An added advantage of the technique is the ability to recover viable cells which have undergone defined numbers of cell divisions by flow cytometric sorting, allowing functional studies to be performed. Other commonly used assays of cell proliferation give only limited information, as they usually measure division at a population level. The CFSE technique can be used to determine kinetics of immune responses, track proliferation in minor subsets of cells and follow the acquisition of differentiation markers or internal proteins linked to cell division. (C) 2000 Elsevier Science B.V.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
ID Code:20332
Year Published:2000
Web of Science® Times Cited:536
Deposited By:Pathology
Deposited On:2000-08-01
Last Modified:2001-05-04

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