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Identification of the Indoleamine 2,3-dioxygenase Nucleotide Sequence in a Rat Liver Transplant Model


Pan, TL and Lin, CL and Chen, CL and Lin, YC and Gojo, S and Lee, TH and Wang, YH and Lord, RJ and Lai, CY and Tsu, LW and Tseng, HP and Wu, ML and Iwashita, Y and Kitano, S and Chiang, KC and Hashimoto, T and Sugioka, A and Goto, S, Identification of the Indoleamine 2,3-dioxygenase Nucleotide Sequence in a Rat Liver Transplant Model, Transplant Immunology, 8, (3) pp. 189-94. ISSN 0966-3274 (2000) [Refereed Article]

DOI: doi:10.1016/S0966-3274(00)00024-1


A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation. © 2000 Elsevier Science B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Transplantation immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Lord, RJ (Dr Roger Lord)
ID Code:20268
Year Published:2000
Web of Science® Times Cited:10
Deposited By:Surgery
Deposited On:2000-08-01
Last Modified:2001-06-08

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