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UV-induced changes in the skin: can they be repaired?


Dandie, GW and Weir, KA and O'Donovan, LA and Muller, HK, UV-induced changes in the skin: can they be repaired?, Redox Report, 5, (2-3) pp. 92-94. ISSN 1351-0002 (2000) [Refereed Article]

DOI: doi:10.1179/135100000101535627


The damaging effects of UVB light have been described previously and include a number of changes to multiple cell types. At previous meetings of this society, we have shown that Langerhans' cells are the most susceptible to UVB induced damage which can be shown as ultrastructural changes in dendrites, nucleus and cytoplasm by transmission electron microscopy. We have also shown that their patterns of migration from skin to regional lymph node and their ability to present antigens to autologous T cells have been profoundly altered by UVB irradiation. The aim of this work was to establish if it was possible to reverse any of the damage done to Langerhans' cells by UVB exposure by topical application of a DNA repair enzyme such as T4N5 endonuclease. These experiments were undertaken in a sheep model that allowed collection of cells as they migrate from the skin. This allowed for a direct examination of the migration characteristics and ultrastructural features of all Langerhans' cells before, during, and for 2 weeks after exposure to a single dose of UVB. Results obtained from this project indicate that treatment by topical application of DNA repair enzyme immediately after UVB irradiation may restore a number of normal immune parameters associated with the structure and function of migrating Langerhans' cells. It appears that there is a dose related correction of the increased tempo of cell migration and some improvements in the number of ultrastructurally damaged Langerhans' cells have also been associated with application of higher doses of DNA repair enzyme. These preliminary findings indicate that some potential therapeutic benefits are associated with the use of such agents in reversing the immunological damage caused by exposure to erythemal doses of UVB light.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Clinical sciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Dandie, GW (Dr Geoffrey Dandie)
UTAS Author:Weir, KA (Ms Kristy Anne Weir)
UTAS Author:O'Donovan, LA (Ms Lisa O'Donovan)
UTAS Author:Muller, HK (Professor Konrad Muller)
ID Code:19880
Year Published:2000
Web of Science® Times Cited:2
Deposited By:Pathology
Deposited On:2000-08-01
Last Modified:2001-05-25

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