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Biotransformation of 1,8-cineole in the brushtail possum (Trichosurus vulpecula)


Boyle, RR and McLean, SR and Davies, NW, Biotransformation of 1,8-cineole in the brushtail possum (Trichosurus vulpecula), Xenobiotica, 30, (9) pp. 915-932. ISSN 0049-8254 (2000) [Refereed Article]

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DOI: doi:10.1080/004982500433336


1. The metabolic fate of 1,8-cineole was investigated in the brushtail possum. Six possums were fed an artificial diet to which 0.5% 1,8-cineole (wet weight) was added for 2 days. Urine and faeces were collected after the second day. A sample of each was extracted into ethyl acetate and analysed for metabolites. Both free and total levels of metabolites were identified by GC-MS and LC-MS and quantified by GC-MS. 2. The pattern of metabolite excretion was very complex in the brushtail possum. Nineteen metabolites were found in total. Metabolites were categorized into four groups according to the oxidation they had undergone: hydroxycineoles (n = 3), cineolic acids (n = 2), dihydroxycineoles (n = 3) and hydroxycineolic acids (n = 11). No hydroxycineolic acid metabolites have been previously reported as metabolites of 1,8-cineole. 3. Fractional recovery of the ingested dose (2.4ą0.5 g; meanąSD) was 0.44ą0.14 (meanąSD) in 24 h. Sixty percent of excreted metabolites were hydroxycineolic acids, the most extensively oxidized metabolites. Conjugation with glucuronic acid was inversely related to metabolite polarity, being greatest for hydroxycineoles (41-82%) and minimal for hydroxycineolic acids. 4. Traces of most metabolites were also found in the faeces.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Basic pharmacology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Terrestrial biodiversity
UTAS Author:Boyle, RR (Dr Rebecca Boyle)
UTAS Author:McLean, SR (Professor Stuart McLean)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
ID Code:19820
Year Published:2000
Web of Science® Times Cited:39
Deposited By:Pharmacy
Deposited On:2000-08-01
Last Modified:2011-05-31

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